1. Volume 134, Issue 7, Pages 2004-2013 (June 2008)
Rikkunshito, an Herbal Medicine, Suppresses Cisplatin-Induced Anorexia in Rats Via 5- HT2 Receptor Antagonism 
Hiroshi Takeda, Chiharu Sadakane, Tomohisa Hattori, Takehiko Katsurada, Tatsuya Ohkawara, Koichi Nagai, Masahiro Asaka 
Gastroenterology 
Volume 134, Issue 7, Pages (June 2008)
DOI: /j.gastro
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2. Figure 1
Plasma acylated- (A and C) or desacylated (B and D) ghrelin levels in cisplatin- or 5-HT–treated rats. Each bar represents the mean ± SE of 8 rats. P values were obtained with Dunnett's test.
Gastroenterology  , DOI: ( /j.gastro )
Copyright © 2008 AGA Institute Terms and Conditions

3. Figure 2
Plasma acylated-ghrelin levels (A), 24-hour food intakes in rats treated with SB or SB HCl in combination with cisplatin (B), and effects on plasma acylated-ghrelin level in sham-operation rats, vagotomized rats, and cisplatin-treated vagotomized rats (C). (A and B) SB and SB HCl were administered intraperitoneally at dose levels of 6 and 10 mg/kg, respectively, 30 minutes before administration of cisplatin. Each bar represents the mean ± SE of 8 rats. (C) Each bar represents the mean ± SE of 8 rats. P values were obtained with Student's t-test or Dunnett's test.
Gastroenterology  , DOI: ( /j.gastro )
Copyright © 2008 AGA Institute Terms and Conditions

4. Figure 3
Plasma acylated- (A) or desacylated (B) ghrelin levels in BW723C86- and mCPP-treated rats, and gastric acylated- or desacylated-ghrelin levels in BW723C86- (C) or mCPP (D) treated rats. The rats fasted for 24 hours, administered BW723C86 (4 or 16 mg/kg) or mCPP (3 or 9 mg/kg), and subjected to blood collection by decapitation at 60 minutes after administration (A and B), and the and removal of the stomach at 60 minutes after administration (D and E). Each bar represents the mean ± SE of 8 rats. P values were obtained with Dunnett's test.
Gastroenterology  , DOI: ( /j.gastro )
Copyright © 2008 AGA Institute Terms and Conditions

5. Figure 4
Plasma acylated-ghrelin levels in 24-hour fasting rats (A) or freely fed rats (B) treated with rikkunshito, 6-hour food intakes in rats treated with rat ghrelin (C) and rats treated with rikkunshito in combination with [D-Lys3]–GHRP-6 (D) in cisplatin-treated rats. (A) Rikkunshito was administered orally at dose levels of 500 or 1000 mg/kg at 16 hours before administration of cisplatin and again simultaneously with administration of cisplatin to 24-hour fasting (A) or free feeding (B) rats. Each bar represents the mean ± SE of 8 rats. (C) Cisplatin was administered intraperitoneally via the tail vein, and after 2 hours, rat acylated-ghrelin was administered intravenously via the tail vein, and food intake was recorded for 4 hours. Each bar represents the mean ± SE of 8 rats. (D) Rikkunshito was administered orally at a dose of 1000 mg/kg at 16 hours before administration of cisplatin and again simultaneously with administration of cisplatin, alone or in combination with [D-Lys3]–GHRP-6. [D-Lys3]–GHRP-6 was administered intraperitoneally simultaneously with cisplatin and the second dose of rikkunshito and the food intake in the subsequent 6 hours was determined. Each bar represents the mean ± SE of 8 rats. P values were obtained with Student's t-test or Dunnett's test.
Gastroenterology  , DOI: ( /j.gastro )
Copyright © 2008 AGA Institute Terms and Conditions

6. Figure 5
Chemical structures of HMF (A), hesperidin (B), isoliquiritigenin (C), and the effect of HMF (D) and hesperidin (E) or isoliquiritigenin (E) on plasma acylated-ghrelin level in cisplatin-treated rats. A single dose of HMF, hesperidin, or isoliquiritigenin was given orally simultaneously with administration of cisplatin. Two hours after the last dose, the rats were subjected to blood collection by decapitation. Each bar represents the mean ± SE of 8 rats. P values were obtained with Student's t-test or Dunnett's test.
Gastroenterology  , DOI: ( /j.gastro )
Copyright © 2008 AGA Institute Terms and Conditions