1. A
PC-9
PC-9/AR B
PC-9/GR
PC-9/GR/AR
AZD9291
AZD9291
AZD9291
AZD9291
CO1686
CO1686
CO1686
CO1686
Erlotinib
Erlotinib
Erlotinib
Erlotinib 25 50 75
100 1 10
1000
10000
Cell number (% of control)
Conc. [nM] 25 50 75
100 1 10
1000
10000
Cell number (% of control)
Conc. [nM] C
Fig. S1. Establishment of AZD9291-resistant cell lines from PC-9 or PC9-GR cells (A and B) and detection of EGFR T797S mutation (C). A and B, The given cell lines were exposed to different concentrations of tested EGFR-TKIs as indicated for 3 days. Cell numbers were estimated with the SRB assay. The data are means ± SDs of four replicate determinations. C, EGFR mutations were detected using ICE COLD-PCR.