Presentation is loading. Please wait.

Presentation is loading. Please wait.

Cold-Temperature Plastic Resin Embedding of Liver for DNA- and RNA-Based Genotyping  Sydney D. Finkelstein, Rajiv Dhir, Mordechai Rabinovitz, Michelle.

Published byRosa Schubert Modified over 6 years ago

Similar presentations

Presentation on theme: "Cold-Temperature Plastic Resin Embedding of Liver for DNA- and RNA-Based Genotyping  Sydney D. Finkelstein, Rajiv Dhir, Mordechai Rabinovitz, Michelle."— Presentation transcript:

1 Cold-Temperature Plastic Resin Embedding of Liver for DNA- and RNA-Based Genotyping 
Sydney D. Finkelstein, Rajiv Dhir, Mordechai Rabinovitz, Michelle Bischeglia, Patricia A. Swalsky, Petrina DeFlavia, Jeffrey Woods, Anke Bakker, Michael Becich  The Journal of Molecular Diagnostics  Volume 1, Issue 1, Pages (November 1999) DOI: /S (10) Copyright © 1999 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

2 Figure 1 Histological appearance of liver biopsy specimens subject to routine (left, 10% neutral buffered formalin/paraffin embedding) and molecular (right, 2% paraformaldehyde/plastic resin embedding) processing. Liver cells fixed and processed routinely manifest homogeneous velvety cytoplasm with round vesicular nucleus including prominent nucleolus. Liver tissue subject to molecular fixation is indistinguishable in histological appearance. Hematoxylin-eosin; original magnifications, ×250 and ×400. The Journal of Molecular Diagnostics 1999 1, 17-22DOI: ( /S (10) ) Copyright © 1999 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

3 Figure 2 Robust amplification of β-actin RNA by RT/PCR (leftmost 8 samples) and epidermal growth factor receptor-2 DNA (rightmost 8 samples) from minute cold-temperature plastic-embedded tissue samples. Microdissected tissue samples consisting of as few as 20 cells from 4-μm-thick histological sections demonstrated strong positive amplification of RNA and DNA gene targets. P, primers only PCR, negative control. The Journal of Molecular Diagnostics 1999 1, 17-22DOI: ( /S (10) ) Copyright © 1999 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

4 Figure 3 RT-PCR amplification of hepatitis C virus for amplicons that are short (left, 5′ nontranslated region, 153 bp) and long (right, NS5 region, 364 bp). Left: All samples subject to molecular fixation and processing yielded strong positive amplification without the requirement for nested primer amplification. All samples routinely fixed failed to effectively amplify consistent with total or nearly total loss of viral RNA. Right: A series of HCV-positive and -negative (N) control samples were subject to RT-PCR for specific detection targeting a 364-bp segment of the NS5 region. P, primers only negative control. The Journal of Molecular Diagnostics 1999 1, 17-22DOI: ( /S (10) ) Copyright © 1999 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

5 Figure 4 HCV genotyping from plastic resin-embedded tissue section. Attainment of abundant PCR product was found to be the critical factor permitting clear and strong DNA sequencing of tissue HCV. Left:Genotyping based on the 5′ nontranslated region reveals type 1 strains in two samples. Right: NS5 region genotyping was preferred due to greater interstrain sequence variation, which in turn permitted detection and characterization of HCV quasispecies. The Journal of Molecular Diagnostics 1999 1, 17-22DOI: ( /S (10) ) Copyright © 1999 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Download ppt "Cold-Temperature Plastic Resin Embedding of Liver for DNA- and RNA-Based Genotyping  Sydney D. Finkelstein, Rajiv Dhir, Mordechai Rabinovitz, Michelle."

Similar presentations

Measurement of Relative Copy Number of CDKN2A/ARF and CDKN2B in Bladder Cancer by Real-Time Quantitative PCR and Multiplex Ligation-Dependent Probe Amplification.

Measurement of Relative Copy Number of CDKN2A/ARF and CDKN2B in Bladder Cancer by Real-Time Quantitative PCR and Multiplex Ligation-Dependent Probe Amplification.
Development of a Novel One-Tube Isothermal Reverse Transcription Thermophilic Helicase-Dependent Amplification Platform for Rapid RNA Detection James Goldmeyer,

Development of a Novel One-Tube Isothermal Reverse Transcription Thermophilic Helicase-Dependent Amplification Platform for Rapid RNA Detection James Goldmeyer,
CyclinD1/CyclinD3 Ratio by Real-Time PCR Improves Specificity for the Diagnosis of Mantle Cell Lymphoma Carol D. Jones, Katherine H. Darnell, Roger A.

CyclinD1/CyclinD3 Ratio by Real-Time PCR Improves Specificity for the Diagnosis of Mantle Cell Lymphoma Carol D. Jones, Katherine H. Darnell, Roger A.
Quantification of Parvovirus B19 DNA Using COBAS AmpliPrep Automated Sample Preparation and LightCycler Real-Time PCR Stefan Schorling, Gunnar Schalasta,

Quantification of Parvovirus B19 DNA Using COBAS AmpliPrep Automated Sample Preparation and LightCycler Real-Time PCR Stefan Schorling, Gunnar Schalasta,
Clinical Laboratory Analysis of Immunoglobulin Heavy Chain Variable Region Genes for Chronic Lymphocytic Leukemia Prognosis  Philippe Szankasi, David.

Clinical Laboratory Analysis of Immunoglobulin Heavy Chain Variable Region Genes for Chronic Lymphocytic Leukemia Prognosis  Philippe Szankasi, David.
Immunoglobulin Gene Mutations and Frequent Use of VH1-69 and VH4-34 Segments in Hepatitis C Virus-Positive and Hepatitis C Virus-Negative Nodal Marginal.

Immunoglobulin Gene Mutations and Frequent Use of VH1-69 and VH4-34 Segments in Hepatitis C Virus-Positive and Hepatitis C Virus-Negative Nodal Marginal.
Myopodin, a Synaptopodin Homologue, Is Frequently Deleted in Invasive Prostate Cancers  Fan Lin, Yan-Ping Yu, Jeff Woods, Kathleen Cieply, Bill Gooding,

Myopodin, a Synaptopodin Homologue, Is Frequently Deleted in Invasive Prostate Cancers  Fan Lin, Yan-Ping Yu, Jeff Woods, Kathleen Cieply, Bill Gooding,
Performance Characteristics of a Quantitative, Homogeneous TaqMan RT-PCR Test for HCV RNA  Joerg Kleiber, Thomas Walter, Gerd Haberhausen, Sue Tsang,

Performance Characteristics of a Quantitative, Homogeneous TaqMan RT-PCR Test for HCV RNA  Joerg Kleiber, Thomas Walter, Gerd Haberhausen, Sue Tsang,
Triple-Negative Breast Cancer

Triple-Negative Breast Cancer
Profiling and Discovery of Novel miRNAs from Formalin-Fixed, Paraffin-Embedded Melanoma and Nodal Specimens  Zhihai Ma, Weng-Onn Lui, Andrew Fire, Soheil.

Profiling and Discovery of Novel miRNAs from Formalin-Fixed, Paraffin-Embedded Melanoma and Nodal Specimens  Zhihai Ma, Weng-Onn Lui, Andrew Fire, Soheil.
Lunx Is a Superior Molecular Marker for Detection of Non-Small Lung Cell Cancer in Peripheral Blood  Michael Mitas, Loretta Hoover, Gerard Silvestri,

Lunx Is a Superior Molecular Marker for Detection of Non-Small Lung Cell Cancer in Peripheral Blood  Michael Mitas, Loretta Hoover, Gerard Silvestri,
Oxidative Activity of the Type 2 Isozyme of 17β-Hydroxysteroid Dehydrogenase (17β- HSD) Predominates in Human Sebaceous Glands  Diane Thiboutot, Patricia.

Oxidative Activity of the Type 2 Isozyme of 17β-Hydroxysteroid Dehydrogenase (17β- HSD) Predominates in Human Sebaceous Glands  Diane Thiboutot, Patricia.
RT-PCR Analysis of RNA Extracted from Bouin-Fixed and Paraffin-Embedded Lymphoid Tissues  Annunziata Gloghini, Barbara Canal, Ulf Klein, Luigino Dal Maso,

RT-PCR Analysis of RNA Extracted from Bouin-Fixed and Paraffin-Embedded Lymphoid Tissues  Annunziata Gloghini, Barbara Canal, Ulf Klein, Luigino Dal Maso,
Volume 61, Issue 2, Pages (February 2002)

Volume 61, Issue 2, Pages (February 2002)
Molecular Diagnosis of Clear Cell Sarcoma

Molecular Diagnosis of Clear Cell Sarcoma
Natalia Kholod, Jacques Boniver, Philippe Delvenne 

Natalia Kholod, Jacques Boniver, Philippe Delvenne 
Molecular Diagnosis of Necrotizing Fasciitis by 16S rRNA Gene Sequencing and Superantigen Gene Detection  Kenneth L. Muldrew, Jean F. Simpson, Charles.

Molecular Diagnosis of Necrotizing Fasciitis by 16S rRNA Gene Sequencing and Superantigen Gene Detection  Kenneth L. Muldrew, Jean F. Simpson, Charles.
Development and Evaluation of a SYBR Green–Based Real-Time Multiplex RT-PCR Assay for Simultaneous Detection and Serotyping of Dengue and Chikungunya.

Development and Evaluation of a SYBR Green–Based Real-Time Multiplex RT-PCR Assay for Simultaneous Detection and Serotyping of Dengue and Chikungunya.
Comparison of Snap Freezing versus Ethanol Fixation for Gene Expression Profiling of Tissue Specimens  Mark A. Perlmutter, Carolyn J.M. Best, John W.

Comparison of Snap Freezing versus Ethanol Fixation for Gene Expression Profiling of Tissue Specimens  Mark A. Perlmutter, Carolyn J.M. Best, John W.
Simple Detection of Telomere Fusions in Pancreatic Cancer, Intraductal Papillary Mucinous Neoplasm, and Pancreatic Cyst Fluid  Tatsuo Hata, Marco Dal.

Simple Detection of Telomere Fusions in Pancreatic Cancer, Intraductal Papillary Mucinous Neoplasm, and Pancreatic Cyst Fluid  Tatsuo Hata, Marco Dal.

Similar presentations

Ads by Google