1. Detection of Biological Material and Integration into Casework Workflow – how to get research implemented into casework
Rachel Fleming
03/05/2018

2. Identifying what is required
Research
Casework and Courts
Implementation into operational casework service delivery

3. Identifying what is required
Research
Casework and Courts
Implementation into operational casework service delivery X

4. Research

5. Research Project Management: Scope Time Cost Quality Human Resources
Risk
Communication
Stakeholders
Procurement

6. Research Casework Project Management: Scope Time Cost Quality
Human Resources
Risk
Communication
Stakeholders
Procurement
Casework

7. Communication Research Casework Project Management: Scope Time Cost
Quality
Human Resources
Risk
Communication
Stakeholders
Procurement
Casework

8. Communication Research Casework Implementation Project Management:
Scope
Time
Cost
Quality
Human Resources
Risk
Communication
Stakeholders
Procurement
Casework
Implementation
Training
SOPs
Health and Safety
Accreditation

9. Next generation of CellTyper
Changes based on:
Casework experience
New research
New equipment/technology (future-proofing)

10. Genetic analyzers
Casework laboratory were starting their transition from 3130xL to 3500xL genetic analyzers

11. Genetic analyzers
CellTyper
CellTyper 2

12. Genetic analyzers CellTyper 2 Research Casework
Not able to do a straightforward comparison of CellTyper vs CellTyper 2.
No RNA experience with the 3500xL
No RNA experience with the 3500xL
CellTyper 2

13. Research MPS research Patricia Albani – PhD project
Stable regions of RNA
Patricia Albani – PhD project
Forensic Sci Int-Gen 20 (2016) 61-70

14. Primer placement – stable RNA region vs non-stable region SLC4A1

15. Primer placement – stable RNA region vs non-stable region SLC4A1

16. CellTyper 2 Duplex (D) Quadruplex (Q) Pentaplex (P) Saliva/ buccal
138 bp
170 bp
Duplex (D)
Circ. blood
M. fluid
Circulatory blood
102 bp
176 bp
Menstrual fluid
105 bp
108 bp
Quadruplex (Q)
Spermatozoa
102 bp
150 bp
Seminal fluid
135 bp
142 bp
Vaginal material
113 bp
Sem.
V. material
Sperm.
Pentaplex (P)

17. Lessons learnt Duplex (D) Quadruplex (Q) Pentaplex (P)
You can never test enough casework-type samples
Always new and different scenarios in casework
Communicate at all times
Understand the workflow in a casework laboratory
Always take longer
Circ. blood
M. fluid
Quadruplex (Q)
Sem.
V. material
Sperm.
Pentaplex (P)

18. Considerations Consumables Implementation 3 multiplex assays Controls
QC of reagents
Number of controls and samples = size of kits required
Need to QC kits of the same lot/batch number
Use kits for the QC of other consumables/steps in the process
QC of reagents
Quality Control

19. Real-time PCR CE
MPS
Different platforms for different laboratories/uses

20. RNA in forensic science at ESR
Casework
Courts
Other laboratories
Companies
Technology
Research
RNA in forensic science at ESR

21. Communication Casework Research Companies Courts Technology
Other laboratories
Companies
Technology
Research
Communication

22. Acknowledgements Patricia Albani Thermo Fisher Scientific
Jayshree Patel
Heidi Baker
SallyAnn Harbison
Johanna Veth
Keshni Rasanayagam
Tracey Haldane
Alicia Haines
“Speaker was provided travel and hotel support by Thermo Fisher Scientific for this presentation, but no remuneration.
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