1. Volume 148, Issue 1, Pages 181-188 (January 2018)
Combining serum microRNA and CA-125 as prognostic indicators of preoperative surgical outcome in women with high-grade serous ovarian cancer 
Jaynish S. Shah, Gregory B. Gard, Jean Yang, Jayne Maidens, Susan Valmadre, Patsy S. Soon, Deborah J. Marsh 
Gynecologic Oncology 
Volume 148, Issue 1, Pages (January 2018)
DOI: /j.ygyno
Copyright © 2017 Elsevier Inc. Terms and Conditions

2. Fig. 1
Identification of markers distinguishing serum samples from women with HGSOC versus healthy volunteer women. Volcano plots showing differentially expressed (DE) markers identified from (A) Training (N=43; 170 miRNAs+CA-125), (B) Test (N=43; 48 miRNAs+CA-125) and (C) Pooled Training+Test sets (N=86; Table 3 and Supplementary Table S1). Vertical and horizontal dashed lines mark the absolute fold-change (log2) of 1.5 and P value (−log10) of 0.05, respectively. Blue and red circles represent predictors with >1.5 absolute fold-change with either unadjusted P<0.05 or were significant after 5% false discovery rate (FDR), respectively. Only ‘red circles’ are annotated with the marker name for simplicity. (D, E) Levels of CA-125 (log2 (U/ml) and candidate miRNAs in Training, Test and the Pooled datasets. The expression of miR-375, miR-210, miR-150-5p and miR-181a-5p are shown in (E) because they were found to be DE after FDR-correction in the pooled dataset. miR-200c-3p was DE only in the training set (Table 3). *P<0.05, **P<0.005 and ***P< Markers that remained significant after FDR-correction are indicated using red asterisks according to the above criteria for significance. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Identification of markers distinguishing serum samples from women with HGSOC versus healthy volunteer women. Volcano plots showing differentially expressed (DE) markers identified from (A) Training (N=43; 170 miRNAs+CA-125), (B) Test (N=43; 48 miRNAs+CA-125) and (C) Pooled Training+Test sets (N=86; Table 3 and Supplementary Table S1). Vertical and horizontal dashed lines mark the absolute fold-change (log2) of 1.5 and P value (−log10) of 0.05, respectively. Volcano plots show open and solid circles, representing predictors circles represent predictors with >1.5 absolute fold-change with either unadjusted P<0.05 or were significant after 5% false discovery rate (FDR), respectively. Only ‘solid circles’ are annotated with the marker name for simplicity. (D, E) Levels of CA-125 (log2 (U/ml) and candidate miRNAs in Training, Test and the Pooled datasets. The expression of miR-375, miR-210, miR-150-5p and miR-181a-5p are shown in (E) because they were found to be DE after FDR-correction in the pooled dataset. miR-200c-3p was DE only in the training set (Table 3). *P<0.05, **P<0.005 and ***P< Markers that remained significant after FDR-correction are indicated using asterisks in parentheses according to the above criteria for significance.
Gynecologic Oncology  , DOI: ( /j.ygyno )
Copyright © 2017 Elsevier Inc. Terms and Conditions

3. Fig. 2
Identification of serum markers that discriminate surgical outcome for women with HGSOC. Volcano plots showing DE markers identified from (A) Training, (B) Test or (C) Pooled (Training+Test set). Vertical and horizontal dashed lines mark the absolute fold-change of 1.5 and P value of 0.05, respectively. Volcano plots show open and solid circles, representing markers with >1.5-fold absolute change with either unadjusted P<0.05 or were significant after 5% false discovery rate (FDR), respectively. Only CA-125, miR-34a-5p and miR-222-3p are labelled for simplicity. (D, E) Levels of CA-125 and candidate miRNAs in Training, Test and the Pooled dataset. miRNAs were selected based on their DE in optimally versus suboptimally cytoreduced serum samples. *P<0.05, **P<0.005 and ***P< Markers that remained significant after FDR-correction are indicated using asterisks in parentheses according to the above criteria for significance.
Gynecologic Oncology  , DOI: ( /j.ygyno )
Copyright © 2017 Elsevier Inc. Terms and Conditions

4. Fig. 3
The effect of hemolysis on DE markers. (A) Hemolysis levels in the three biological groups (Healthy, Optimally and Suboptimally cytoreduced) measured by the ‘miR ratio’ (delta Cq (miR-23a-3p—miR-451a)). (B–F) The effect of hemolysis on candidate miRNAs and CA-125. miR-210 was significantly affected by hemolysis **P<0.005.
Gynecologic Oncology  , DOI: ( /j.ygyno )
Copyright © 2017 Elsevier Inc. Terms and Conditions