1. DNA Sequencing A population of DNA fragments is generated.
One end is common to all fragments (the 5’ end of the sequencing primer).
The other end terminates at all possible positions (the 3- terminus).

2. 2',3'-Dideoxyribonucleoside Triphosphates

3. Sanger Sequencing

6. Automated DNA Sequencing
Fluorescent dyes are used for detection of DNA chains instead of radioactive isotopes.
Products of all four chain terminator reactions are separated through a single gel or capillary tube.
Photocells detect fluorescence of the dyes as they pass through the gel or capillary tube.
Output of the photocell is directly transferred to a computer for analysis.

18. Next Gen