1. Altogen labs
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > AR42J Transfection Reagent (Pancreatic Carcinoma Cells, CRL-1492)
Altogen Biosystems offers the AR42J Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits.
AR42J Transfection Reagent is an advanced formulation of lipid-based reagents, and it is developed to provide high transfection efficiency with AR42J and other pancreas derived cancer cell lines.
This cell line is an excellent host for studying pancreatic cancer and other cancer types. When cultured in vitro, AR42J cells are a monolayer and adhere to the surface of the culture flask.
Other applications include drug discovery, gene expression studies, molecular and cell biology research applications.
Purchase AR42J Transfection Kit at
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
Telephone   Fax   

2. AR42J Cell Line Description and Applications
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > AR42J Transfection Reagent (Pancreatic Carcinoma Cells, CRL-1492)
AR42J Cell Line Description and Applications
The AR42J cell line originates from the pancreatic tissue of a Wistar rat (Rattus norvegicus) that had a pancreatic tumor. The cell line has receptors for glucocorticoid and insulin. The AR42J cell is tumorigenic and can be used as a transfection host. It displays epithelial morphology and produces amylase among other exocrine enzymes. This production can be facilitated through the receptors mentioned above. Furthermore, the secretory activity that can be induced by glucocorticoid stimulation is paralleled with an involved reorganization of the Endoplasmic Reticulum.
AR42J Cell Line
Atcc.org
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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3. AR42J Transfection Protocol
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > AR42J Transfection Reagent (Pancreatic Carcinoma Cells, CRL-1492)
AR42J Transfection Protocol
1. Plate 10, ,000 AR42J cells per well in 0.5 ml of complete growth medium 12–24 hours prior to transfection
2. Wash with 1xPBS and add 0.5 ml of fresh growth medium
3. Prepare transfection complexes by mixing 40 µl of serum-free medium, 5.5 µl of transfection reagent, and
• 750 ng DNA (or mRNA), or
• 30 nM - 50 nM of siRNA (or microRNA)
*Referred to a final volume including growth medium
4. Incubate transfection complexes at RT for minutes
5. Optional: Add 2 µl of Complex Condenser. This reagent reduces the size of transfection complex, therefore increasing transfection efficiency; however, it may increase cell toxicity
6. Add prepared transfection complexes to 0.5 ml of complete growth medium with AR42J cells (from step 2)
7. Incubate cells at 37ºC in a humidified CO2 incubator
8. Assay for phenotype or target gene expression hours after transfection
Optional: Transfection efficiency can be increased by addition of Transfection Enhancer reagent. Add 2 µl of Transfection Enhancer reagent hours after transfection
If the viability of AR42J cells being transfected is affected at hours post-transfection, the level of cytotoxicity can be decreased by changing the growth medium and eliminating redundant exposure of cells to transfectant
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
Telephone   Fax   

4. General Lipoplex-mediated Transfection Mechanism of Action
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > AR42J Transfection Reagent (Pancreatic Carcinoma Cells, CRL-1492)
General Lipoplex-mediated Transfection Mechanism of Action
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
Telephone   Fax   

5. AR42J Transfection Kit Product Details
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > AR42J Transfection Reagent (Pancreatic Carcinoma Cells, CRL-1492)
AR42J Transfection Kit Product Details
Two-component formulation enhances lipid-mediated transfection efficiency
Optimized easy-to-use transfection protocol provided for transfection of siRNA, DNA, mRNA, and microRNA
Kit includes Transfection Enhancer reagent and recommended transfection protocol
High transfection efficacy in the presence of serum
Expand your RNAi application with a reagent optimized for delivery of both siRNA and plasmid
Reproducible transfection results
Works well for standard reverse transfection and high-throughput applications
Developed and manufactured by Altogen Biosystems (
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
Telephone   Fax   

6. Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > AR42J Transfection Reagent (Pancreatic Carcinoma Cells, CRL-1492)
Data
Figure 1. Cyclophilin B silencing efficiency was determined by qRT-PCR in the AR42J cells transfected by Cyclophilin B siRNA or non-silencing siRNA control following the recommended transfection protocol. Cyclophilin mRNA expression levels were measured 48 hours post-transfection. 18S rRNA levels were used to normalize the Cyclophilin B data. Values are normalized to untreated sample. Data are presented as means ± SD (n=6).

7. Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > AR42J Transfection Reagent (Pancreatic Carcinoma Cells, CRL-1492)
Data
Figure 2. Protein expression of Cyclophilin B in AR42J cells. DNA plasmid expressing Cyclophilin B or siRNA targeting Cyclophilin B were transfected into AR42J cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.

8. AR42J Transfection Kit Benefits
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > AR42J Transfection Reagent (Pancreatic Carcinoma Cells, CRL-1492)
AR42J Transfection Kit Benefits
Pre-optimized transfection protocol for AR42J cell line
Compatible with DNA, small RNA (siRNA, shRNA, miRNA), mRNA, and small protein complexing
Free of serum and protein of animal origin
Compatible with standard and reverse transfection methods (both protocols provided in the kit manual)
Easy to use AR42J transfection protocol ensures great performance with expedited experimental timeline
Equally efficient for single or multiple transfections
Can be used for transient transfection and development of stable AR42J cell lines
Bio-degradable after endocytosis
Used for preclinical research worldwide
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
Telephone   Fax   