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Anti–4-1BB/PD-1 combination enriched CD8+ T cells in TILs

Published byMaría José Gil Navarro Modified over 6 years ago

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Presentation on theme: "Anti–4-1BB/PD-1 combination enriched CD8+ T cells in TILs"— Presentation transcript:

1 Anti–4-1BB/PD-1 combination enriched CD8+ T cells in TILs
Anti–4-1BB/PD-1 combination enriched CD8+ T cells in TILs. Tumor fragments were harvested from B16F10 tumor–bearing mice after antibody treatment and disaggregated into a single-cell suspension. Anti–4-1BB/PD-1 combination enriched CD8+ T cells in TILs. Tumor fragments were harvested from B16F10 tumor–bearing mice after antibody treatment and disaggregated into a single-cell suspension. After surface staining, cells were fixed and permeabilized for intracellular marker staining, and finally analyzed by flow cytometry for the expression of CD3, CD4, CD8, CD25, KLRG1, and Foxp3. A, the ratio of CD8+ to CD4+ T cells; B, the frequency of Treg (CD4+CD25+Foxp3+); C, the ratio of CD8+ T cells to Treg; and D, the expression of KLRG1 on CD8+ T cells. ***, P < and ****, P < , when comparing groups as indicated by the horizontal lines. Shihao Chen et al. Cancer Immunol Res 2015;3: ©2015 by American Association for Cancer Research

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