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Streptavidin on the cell surface

Published byGiovanna Barreiro Pereira Modified over 6 years ago

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Presentation on theme: "Streptavidin on the cell surface"— Presentation transcript:

1 Streptavidin on the cell surface
Binds strongly to biotin molecule Used to bind biotinylated nucleic acids or peptides Big idea: Streptavidin protein expressed on the cell surface can be used to target any biotinylated DNA/protein to the cell surface. McDevitt, 1999

2 Lpp-OmpA surface display
J. Francisco, C. Earhart, G. Georgiou, 1992 periplasm extracellular outer membrane Surface protein Lpp (lipoprotein) signal peptide OmpA (outer membrane protein A) transmembrane domains (1 or 5)

3 BioBricks assembly of protein domains
I. Phillips P. Silver 2006 5’…TCTAGAG XbaI TACTAGT…3’ SpeI Standard BioBrick 5’…TCTAGA_ XbaI _ACTAGT…3’ SpeI Protein domain BioBrick TCTAGT Mixed 6-bp mixed site Reading frame maintained Promoter RBS Lpp OmpA Streptavidin O1 SM (Ting) O5 SD (Aslan)

4 Expression of construct
Anti-His L-O1-SM L-O5-SD L-O1-SD L-O5-SM L-O1-SM Anti-Streptavidin L-O5-SD L-O1-SD L-O5-SM

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