1. ENTEROBACTERIACEAE (Klebsiella, Salmonella, Shigella)
Prepared by:
Miss Norzawani Jaffar
Bsc (Hons) Biomedical Sciences, UKM

2. Learning Outcome
At the end of learning process student must be able to;
Describe characteristic of Enterobactericeae
Describe the specific characteristic for Klebsiella, Salmonella and Shigella
Understand the role in disease for Klebsiella, Salmonella and Shigella.
Explain the significant laboratory diagnosis for Klebsiella, Salmonella and Shigella identification.

3. Characteristic

5. Klebsiella

6. Salmonella

7. Shigella

8. Confirmatory test
Microscopy of stool samples show manu white blood cells but few if any organism
Culture and biochemistry show no motility, no gas from glucose and no lactose fermentation.
Serology distinguishes serotypes of all except S. sonnei.

10. Laboratory Diagnosis

13. Laboratory Diagnosis

15. In most laboratories a metronidazole antibiotic disc is placed at the juction of the primary and second streak of the anaerobic blood plates (78a).
The plate is incubated in an anaerobic cabinet and examined at disc identifies anaerobes, and a report is issued stating ‘anaerobes present; metronidazole sensitive’ (78b).

17. End………. q & a??