1. Evaluation of a disposable plastic Neubauer counting chamber for semen analysis 
Jackson Kirkman-Brown, Ph.D., Lars Björndahl, M.D., Ph.D. 
Fertility and Sterility 
Volume 91, Issue 2, Pages (February 2009)
DOI: /j.fertnstert
Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
Individual results from 18 consecutive samples in glass and plastic chambers, respectively (series 1). Left: pairwise scatter diagram; right: Bland-Altman plot showing systematic error.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

3. Figure 2
Results from multiple assessments of different sperm dilutions. In series 2 (n = 7) and series 3 (n = 5) significantly lower concentrations (a: P<.05) were obtained with the plastic chambers. In series 4 (n = 5) a microscope with high-quality optics was used (Olympus BX50), and efforts were made to trace sperm that could be hidden because of optical phenomena (b: P>.05). In this last series no difference was observed between the two types of chambers.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

4. Figure 3
(A): Field from standard glass counting chamber (improved Neubauer pattern). Photomicrograph from Nikon Eclipse E600 microscope (objective: Plan Fluor ×10/0.25, Ph1 DL ∞/0.17 WD 16; intermediate magnification (tube factor): ×2) was used for photomicrography (Sony SSC-DC58AP color video camera; image capture with Picsara 8.81 rev. 2). (B, C): Field from plastic disposable counting chamber (improved Neubauer pattern) at different levels of focal depth. Arrow points at a position where a sperm head is hidden on a line in the chamber and therefore difficult to locate and identify. Same equipment for photography as for (A).
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions