Presentation is loading. Please wait.

Presentation is loading. Please wait.

Inhibition of EGFR Tyrosine Kinase by Erlotinib Prevents Sclerodermatous Graft-Versus- Host Disease in a Mouse Model  Florence Morin, Niloufar Kavian,

Published byÉmile Bordeleau Modified over 6 years ago

Similar presentations

Presentation on theme: "Inhibition of EGFR Tyrosine Kinase by Erlotinib Prevents Sclerodermatous Graft-Versus- Host Disease in a Mouse Model  Florence Morin, Niloufar Kavian,"— Presentation transcript:

1 Inhibition of EGFR Tyrosine Kinase by Erlotinib Prevents Sclerodermatous Graft-Versus- Host Disease in a Mouse Model  Florence Morin, Niloufar Kavian, Wioleta Marut, Christiane Chéreau, Olivier Cerles, Philippe Grange, Bernard Weill, Carole Nicco, Frédéric Batteux  Journal of Investigative Dermatology  Volume 135, Issue 10, Pages (October 2015) DOI: /jid Copyright © 2015 The Society for Investigative Dermatology, Inc Terms and Conditions

2 Figure 1 Effects of Erlotinib on mouse ear skin following graft-versus-host disease (GVHD) induction and oral administration of Erlotinib (Erlo). (a) Immunohistochemical study of phospho-EGFR (pEGFR) on 7 μm ear skin sections in syngeneic (Syn) BALB/c mice and in GVHD mice, revealed with diaminobenzidine tetrahydrochloride. (b) Western blot of pEGFR expression in the ear skin of BALB/c mice submitted to a syngeneic or a nonsyngeneic graft. Photographs were taken with a Nikon Eclipse 80i microscope. Original magnification × 50. Values in b are mean±SEM. *P≤0.05; ***P≤ AU, arbitrary unit. Journal of Investigative Dermatology  , DOI: ( /jid ) Copyright © 2015 The Society for Investigative Dermatology, Inc Terms and Conditions

3 Figure 2 Effects of Erlotinib (Erlo) on clinical features following graft-versus-host disease (GVHD) induction and oral administration of Erlotinib. (a) Evolution of the GVHD score in the different groups during Erlotinib treatment. (b) Evolution of ear skin thickness during Erlotinib treatment. (c) Representative Sirius red–dyed ear skin sections of 7 μm, showing fibrosis in syngeneic (Syn) BALB/c mice and in GVHD mice, treated by Erlotinib or not. Photographs were taken with a Nikon Eclipse 80i microscope. Original magnification × 50. Values in a and b are mean±SEM. *P≤0.05; ***P≤ Bars represent mean±SEM. AU, arbitrary unit. Journal of Investigative Dermatology  , DOI: ( /jid ) Copyright © 2015 The Society for Investigative Dermatology, Inc Terms and Conditions

4 Figure 3 Effects of Erlotinib (Erlo) on mouse skin following graft-versus-host disease (GVHD) induction and oral administration of Erlotinib. (a) Representative 5 μm frozen skin sections with enhanced green fluorescent protein (eGFP) donor cells and phycoerythrin-conjugated anti-CD3 mAb staining. (b) Scores obtained by enumerating eGFP- and anti-CD3-positive cells in 10 areas per frozen skin section. (c) Levels of IFN-γ and IL-13 produced by skin cells and used as markers of T helper type 1 and 2 (Th1 and Th2) responses. (d) Levels of mRNAs for IFN-γ, IL-13, CCL17, CCL21, and CCL27 in the skin. Photographs were taken with a Nikon Eclipse 80i microscope. Original magnification: × 100. Values in b, c, and d are mean±SEM. *P≤0.05; **P≤0.01; ***P≤ qRT-PCR, quantitative real-time reverse-transcriptase–PCR; Syn, syngeneic. Journal of Investigative Dermatology  , DOI: ( /jid ) Copyright © 2015 The Society for Investigative Dermatology, Inc Terms and Conditions

5 Figure 4 Effects of Erlotinib (Erlo) on liver involvement following graft-versus-host disease (GVHD) induction and oral administration of Erlotinib. (a) Hematoxylin–eosin staining of liver sections. GVH and GVH Erlo groups showed mixed lymphomonocytic infiltrations around the portal vein with a major reduction in the GVH Erlo group. (b) Serum aspartate aminotransferase (AST) levels in various experimental groups. (c) Serum alanine aminotransferase (ALT) levels in various experimental groups. Photographs were taken using a Nikon Eclipse 80i microscope. The arrows showed mixed lymphocytic infiltrations around the portal vein. Original magnification × 100. Values in b and c are mean±SEM. *P≤0.05. Syn, syngeneic. Journal of Investigative Dermatology  , DOI: ( /jid ) Copyright © 2015 The Society for Investigative Dermatology, Inc Terms and Conditions

6 Figure 5 Inhibition of the production of autoantibodies in mice with graft-versus-host disease (GVHD) systemic sclerosis treated in vivo with Erlotinib (Erlo). Inhibition of the production of T cells with sclerodermatous GVHD (Scl-GVHD) treated in vivo with Erlotinib. (a) Levels of anti-topoisomerase I antibodies (Abs), as measured by ELISA. (b) Splenic B-cell numbers, as assessed by flow cytometry. (c) Splenic CD4 T-cell numbers, as assessed by flow cytometry. (d) Splenic CD8 T-cell numbers, as assessed by flow cytometry. (e) Ratio between naive and memory CD4 cells. (f) Ratio between naive and memory CD8 cells. Values in a–f are mean±SEM. *P≤0.05; ***P≤ AU, arbitrary unit; NS, nonsignificant; Syn, syngeneic. Journal of Investigative Dermatology  , DOI: ( /jid ) Copyright © 2015 The Society for Investigative Dermatology, Inc Terms and Conditions

Download ppt "Inhibition of EGFR Tyrosine Kinase by Erlotinib Prevents Sclerodermatous Graft-Versus- Host Disease in a Mouse Model  Florence Morin, Niloufar Kavian,"

Similar presentations

IL-18 Downregulates Collagen Production in Human Dermal Fibroblasts via the ERK Pathway  Hee Jung Kim, Seok Bean Song, Jung Min Choi, Kyung Moon Kim,

IL-18 Downregulates Collagen Production in Human Dermal Fibroblasts via the ERK Pathway  Hee Jung Kim, Seok Bean Song, Jung Min Choi, Kyung Moon Kim,
The Tumor Necrosis Factor Superfamily Molecule LIGHT Promotes Keratinocyte Activity and Skin Fibrosis  Rana Herro, Ricardo Da S. Antunes, Amelia R. Aguilera,

The Tumor Necrosis Factor Superfamily Molecule LIGHT Promotes Keratinocyte Activity and Skin Fibrosis  Rana Herro, Ricardo Da S. Antunes, Amelia R. Aguilera,
Loss of Extracellular Superoxide Dismutase Induces Severe IL-23-Mediated Skin Inflammation in Mice  Yun Sang Lee, In-Su Cheon, Byung-Hak Kim, Myung-Ja.

Loss of Extracellular Superoxide Dismutase Induces Severe IL-23-Mediated Skin Inflammation in Mice  Yun Sang Lee, In-Su Cheon, Byung-Hak Kim, Myung-Ja.
Identification of CD3+CD4−CD8− T Cells as Potential Regulatory Cells in an Experimental Murine Model of Graft-Versus-Host Skin Disease (GVHD)  Fumi Miyagawa,

Identification of CD3+CD4−CD8− T Cells as Potential Regulatory Cells in an Experimental Murine Model of Graft-Versus-Host Skin Disease (GVHD)  Fumi Miyagawa,
Cutaneous RANK–RANKL Signaling Upregulates CD8-Mediated Antiviral Immunity during Herpes simplex Virus Infection by Preventing Virus-Induced Langerhans.

Cutaneous RANK–RANKL Signaling Upregulates CD8-Mediated Antiviral Immunity during Herpes simplex Virus Infection by Preventing Virus-Induced Langerhans.
Latency-Associated Peptide Prevents Skin Fibrosis in Murine Sclerodermatous Graft- Versus-Host Disease, a Model for Human Scleroderma  Yan Zhang, Laura.

Latency-Associated Peptide Prevents Skin Fibrosis in Murine Sclerodermatous Graft- Versus-Host Disease, a Model for Human Scleroderma  Yan Zhang, Laura.
Induction of heme oxygenase-1 before conditioning results in improved survival and reduced graft-versus-host disease after experimental allogeneic bone.

Induction of heme oxygenase-1 before conditioning results in improved survival and reduced graft-versus-host disease after experimental allogeneic bone.
Preventive Azithromycin Treatment Reduces Noninfectious Lung Injury and Acute Graft- versus-Host Disease in a Murine Model of Allogeneic Hematopoietic.

Preventive Azithromycin Treatment Reduces Noninfectious Lung Injury and Acute Graft- versus-Host Disease in a Murine Model of Allogeneic Hematopoietic.
Mesenchymal Stem Cells (MSCs) Attenuate Cutaneous Sclerodermatous Graft-Versus- Host Disease (Scl-GVHD) through Inhibition of Immune Cell Infiltration.

Mesenchymal Stem Cells (MSCs) Attenuate Cutaneous Sclerodermatous Graft-Versus- Host Disease (Scl-GVHD) through Inhibition of Immune Cell Infiltration.
CXCR3-Mediated Skin Homing of Autoreactive CD8 T Cells Is a Key Determinant in Murine Graft-Versus-Host Disease  Vadim A. Villarroel, Naoko Okiyama, Gaku.

CXCR3-Mediated Skin Homing of Autoreactive CD8 T Cells Is a Key Determinant in Murine Graft-Versus-Host Disease  Vadim A. Villarroel, Naoko Okiyama, Gaku.
The Antifibrotic Effect of α2AP Neutralization in Systemic Sclerosis Dermal Fibroblasts and Mouse Models of Systemic Sclerosis  Yosuke Kanno, En Shu,

The Antifibrotic Effect of α2AP Neutralization in Systemic Sclerosis Dermal Fibroblasts and Mouse Models of Systemic Sclerosis  Yosuke Kanno, En Shu,
Kruppel-Like Factor KLF4 Facilitates Cutaneous Wound Healing by Promoting Fibrocyte Generation from Myeloid-Derived Suppressor Cells  Lingling Ou, Ying.

Kruppel-Like Factor KLF4 Facilitates Cutaneous Wound Healing by Promoting Fibrocyte Generation from Myeloid-Derived Suppressor Cells  Lingling Ou, Ying.
Type I IL-1 Receptor Mediates IL-1 and Intracellular IL-1 Receptor Antagonist Effects in Skin Inflammation  Gaby Palmer, Dominique Talabot-Ayer, Gürkan.

Type I IL-1 Receptor Mediates IL-1 and Intracellular IL-1 Receptor Antagonist Effects in Skin Inflammation  Gaby Palmer, Dominique Talabot-Ayer, Gürkan.
Induction of Autoimmunity in a Bleomycin-Induced Murine Model of Experimental Systemic Sclerosis: An Important Role for CD4+ T Cells  Hideaki Ishikawa,

Induction of Autoimmunity in a Bleomycin-Induced Murine Model of Experimental Systemic Sclerosis: An Important Role for CD4+ T Cells  Hideaki Ishikawa,
Toll-Like Receptor-4 Deficiency Enhances Repair of UVR-Induced Cutaneous DNA Damage by Nucleotide Excision Repair Mechanism  Israr Ahmad, Eva Simanyi,

Toll-Like Receptor-4 Deficiency Enhances Repair of UVR-Induced Cutaneous DNA Damage by Nucleotide Excision Repair Mechanism  Israr Ahmad, Eva Simanyi,
Volume 82, Issue 1, Pages (July 2012)

Volume 82, Issue 1, Pages (July 2012)
IL-6 Blockade Attenuates the Development of Murine Sclerodermatous Chronic Graft- Versus-Host Disease  Doanh Le Huu, Takashi Matsushita, Guihua Jin, Yasuhito.

IL-6 Blockade Attenuates the Development of Murine Sclerodermatous Chronic Graft- Versus-Host Disease  Doanh Le Huu, Takashi Matsushita, Guihua Jin, Yasuhito.
Oxidation of Cell Surface Thiol Groups by Contact Sensitizers Triggers the Maturation of Dendritic Cells  Saori Kagatani, Yoshinori Sasaki, Morihiko Hirota,

Oxidation of Cell Surface Thiol Groups by Contact Sensitizers Triggers the Maturation of Dendritic Cells  Saori Kagatani, Yoshinori Sasaki, Morihiko Hirota,
Receptor Activator of NF-κB Ligand Promotes the Production of CCL17 from RANK+ M2 Macrophages  Taku Fujimura, Yumi Kambayashi, Sadanori Furudate, Masayuki.

Receptor Activator of NF-κB Ligand Promotes the Production of CCL17 from RANK+ M2 Macrophages  Taku Fujimura, Yumi Kambayashi, Sadanori Furudate, Masayuki.
Volume 44, Issue 6, Pages (June 2006)

Volume 44, Issue 6, Pages (June 2006)

Similar presentations

Ads by Google