1. Cdk1 inhibition does not affect kinetochore capturing.
Cdk1 inhibition does not affect kinetochore capturing. (A) cdc28‐as1 GAL‐CENIII–GFP SPC42–RFP MET‐CDC20 cells were incubated in nocodazole‐containing YEP+Raff+Gal+Met medium and then released into three different media conditions: (1) YEP+Gal+Met+1NM‐PP1 medium, (2) YEP+Glu+Met+1NM‐PP1 medium and (3) YEP+Glu+Met−1NM‐PP1 medium. Photomicrographs depict the positions of CENIII–GFP and Spc42–RFP 30 min after release. Bar scale: 5 μm. Bottom panels: bar charts showing proportion of cells with distances between CENIII–GFP and Spc42–RFP after release from nocodazole arrest. Approximately 100 cells were analysed for each sample. (B) Bi‐orientation in cdc28‐as1 slk19Δ cdc15‐2 cells after release from HU arrest in absence or presence of 1NM‐PP1. Photomicrographs show the state of nuclear division, spindle staining and CENV–GFP signal 60 and 120 min after release from HU arrest. Bar scale: 5 μm. Graph depicts bi‐orientation kinetics after the release. Experiments were performed twice and >200 cells were analysed for each sample.
Hongqing Liang et al. EMBO J. 2012;31:
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