1. Tertiary butyl hydroperoxide induced oxidative damage in mice erythrocytes: Protection by taurine 
Anandita Roy, Parames C. Sil 
Pathophysiology 
Volume 19, Issue 2, Pages (April 2012)
DOI: /j.pathophys
Copyright © 2012 Elsevier Ireland Ltd Terms and Conditions

2. Fig. 1
(A) Increase in intracellular ROS in erythrocytes with increase in concentration of TBHP (1, 3, 4, 5, 7 and 8μM, respectively). (B) Increase in ROS production in erythrocytes on treatment with 5μM TBHP, with increase in time (0–30min). (C) Decrease in ROS production in erythrocytes on treatment with 5μM TBHP, with increasing concentration of taurine. Each point or column indicate±SD, n=6. Data were analyzed by one-way ANOVA, with Student–Newman–Keuls post hoc tests. Differences were attributed at P<0.05 and homogeneous subgroups share common superscripted letters.
Pathophysiology  , DOI: ( /j.pathophys )
Copyright © 2012 Elsevier Ireland Ltd Terms and Conditions

3. Fig. 2
(A) Leakage of hemoglobin from erythrocytes on treatment with TBHP and subsequent protection by taurine. (B) The amount of ROS present in normal and experimental erythrocytes. (C) Formation of methemoglobin in normal and treated erythrocytes. Cont: normal or untreated erythrocytes; TBHP: erythrocytes treated with TBHP (toxin control); Tau+TBHP: erythrocytes treated with taurine prior to TBHP intoxication; Tau & TBHP: erythrocytes treated with TBHP and taurine simultaneously. Each point or column indicate±SD, n=6. Data were analyzed by one-way ANOVA, with Student–Newman–Keuls post hoc tests. Differences were attributed at P<0.05 and homogeneous subgroups share common superscripted letters.
Pathophysiology  , DOI: ( /j.pathophys )
Copyright © 2012 Elsevier Ireland Ltd Terms and Conditions

4. Fig. 3
Change in calpain, calcium dependent protease in erythrocytes treated with TBHP by Western blot analysis. Left panel represents Western blot analysis and the right panel is the quantification of bands. β-Actin was used as internal control. The relative intensities of bands were determined using NIH-image software and the control band was given an arbitrary value of 1 (100%). Cont: normal or untreated erythrocytes; TBHP: erythrocytes treated with TBHP (toxin control); Tau+TBHP: erythrocytes treated with taurine prior to TBHP intoxication; Tau & TBHP: erythrocytes treated with TBHP and taurine simultaneously. Each point or column indicate±SD, n=6. Data were analyzed by one-way ANOVA, with Student–Newman–Keuls post hoc tests. Differences were attributed at P<0.05 and homogeneous subgroups share common superscripted letters.
Pathophysiology  , DOI: ( /j.pathophys )
Copyright © 2012 Elsevier Ireland Ltd Terms and Conditions

5. Fig. 4
FACS analysis of shape changes and phosphatidylserine exposure of normal and experimental erythrocytes. (A) Enhanced forward scatter of shrunken erythrocytes with an increase in density and decreased cell volume. (B) Distribution of FITC-Annexin-V labeled erythrocytes. Cont: Normal or untreated erythrocytes; TBHP: erythrocytes treated with TBHP (toxin control); Tau+TBHP: erythrocytes treated with taurine prior to TBHP intoxication; Tau & TBHP: erythrocytes treated with TBHP and taurine simultaneously.
Pathophysiology  , DOI: ( /j.pathophys )
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6. Fig. 5
FACS analysis of phosphatidylserine exposure of normal and experimental erythrocytes shows distribution of FITC-Annexin-V labeled erythrocytes. (A) Erythrocytes pretreated with β-alanine, followed by TBHP and taurine. Annexin-V positive erythrocytes did not decrease on taurine treatment. (B) Normal erythrocytes treated with TBHP and taurine. Cont: normal or untreated erythrocytes; TBHP: erythrocytes treated with TBHP (toxin control); Tau+TBHP: erythrocytes treated with taurine prior to TBHP intoxication; Tau & TBHP: erythrocytes treated with TBHP and taurine simultaneously.
Pathophysiology  , DOI: ( /j.pathophys )
Copyright © 2012 Elsevier Ireland Ltd Terms and Conditions

7. Fig. 6
FACS analysis of normal and experimental erythrocytes. (A) ROS formation upon TBHP exposure on β-alanine pretreated erythrocytes. ROS production was not attenuated on treatment with taurine. (B) ROS formation of normal erythrocytes upon TBHP exposure which was effectively curbed on taurine treatment. Cont: normal or untreated erythrocytes; TBHP: erythrocytes treated with TBHP (toxin control); Tau+TBHP: erythrocytes treated with taurine prior to TBHP intoxication; Tau & TBHP: erythrocytes treated with TBHP and taurine simultaneously.
Pathophysiology  , DOI: ( /j.pathophys )
Copyright © 2012 Elsevier Ireland Ltd Terms and Conditions

8. Fig. 7
The effect of different taurine concentrations on attenuation of ROS in β-alanine pretreated erythrocytes, formed upon TBHP exposure. Cont: normal or untreated erythrocytes; TBHP: erythrocytes treated with TBHP (toxin control); Tau+TBHP: erythrocytes treated with taurine prior to TBHP intoxication; Tau & TBHP: erythrocytes treated with TBHP and taurine simultaneously. Each point or column indicate±SD, n=6. Data were analyzed by one-way ANOVA, with Student–Newman–Keuls post hoc tests. Differences were attributed at P<0.05 and homogeneous subgroups share common superscripted letters.
Pathophysiology  , DOI: ( /j.pathophys )
Copyright © 2012 Elsevier Ireland Ltd Terms and Conditions

9. Fig. 8
Increase in intracellular Ca2+ in erythrocytes exposed to TBHP, BAPTA-AM and taurine, by the Ca2+ binding fluorescent probe Fura 2. Cont: normal or untreated erythrocytes; TBHP: erythrocytes treated with TBHP (toxin control); Tau+TBHP: erythrocytes treated with taurine prior to TBHP intoxication; Tau & TBHP: erythrocytes treated with TBHP and taurine simultaneously, Tau:erythrocytes treated with taurine only, BAPTA-AM: erythrocytes treated with BAPTA-AM only, TBHP+BAPTA-AM: erythrocytes treated with TBHP and BAPTA-AM and TBHP+BAPTA-AM+TAU: erythrocytes treated with TBHP, BAPTA-AM and taurine together.
Pathophysiology  , DOI: ( /j.pathophys )
Copyright © 2012 Elsevier Ireland Ltd Terms and Conditions

10. Fig. 9
SEM photographs of erythrocytes. 1: normal or untreated erythrocytes; 2: erythrocytes treated with TBHP, arrows indicate echinocytes; 3: closeup of echinocyte, arrows indicate blebs or spicules; 4: erythrocytes treated with taurine prior to TBHP intoxication; 5: erythrocytes treated with TBHP and taurine simultaneously.
Pathophysiology  , DOI: ( /j.pathophys )
Copyright © 2012 Elsevier Ireland Ltd Terms and Conditions