1. Group Name
Ecoli2
Group member name

2. Isolates Sample name Country Collection date ec11-4623 France ec
c227-11
Germany ec
2011 ec
2004

3. Result of species identification (KmerFinder)
Strain
Species acc. to Grad et al 2012
Predicted species
Closest matching strain
Template coverage ec ec
c227-11 ec ec

4. Representative KmerFinder result

5. Result of Multilocus Sequence Typing (MLST)
Strain
ST acc. to Grad et al 2012
Predicted ST ec ec
c227-11 ec ec

6. Representative Multilocus Sequence Typing result

7. Result of serotyping ec11-4623 ec11-5537 c227-11 ec11-3798 ec04-8351
Strain
Serotype acc. to Grad et al 2012
Predicted serotype ec ec
c227-11 ec ec

8. Representative serotyping result

9. Result of antibiotics resistance analysis
Isolate
Ampicillin (blaZ, blaTem-1B)
Third-generation cephalosporins (blaCTX-M-15)
Streptomycin (strA, strB, aadA)
Nalidixic acid (chromosomal mutations)
Tetracycline (tetC, tetM, tetR, tetS, tetA) ec ec
c227-11 ec ec
Isolate
Cotri-moxazole (dfr)
Carbape-nems (blaNDM)
Ciprofloxacin (chromosomal mutations)
Chloram-phenicol (cat)
Kanamycin (aph(3’)-II/aph(3’)-III)
Gentamicin (aac(6')-aph(2'')) ec ec
c227-11 ec ec
How does it compare to the results presented in Grad et al 2012?

10. Virulence factors Isolate stx2 (stx2a variant) aggR pic stx1 eae hlyA
astA
papG
dsbD ec ec
c227-11 ec ec
Indicate with , e.g., a “X”, if the gene was present or not
Which pathovar does the outbreak isolates seem to belong to?

11. Result of plasmid analysis
Isolate
IncFII
IncFIB
IncI1
IncP
IncQ1
IncB/O/K/Z ec ec
c227-11 ec ec
For each strain write which node the replicon was found on. If not found, leave the cell empty.
Which plasmid is the blaCTX-M-15 gene on?
Which plasmid are the aggR gene on?

12. Distance matrix made by NDtree and tree made with neighbour-joining

13. Distance matrix made by CSIPhylogeny and tree made with neighbour-joining