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Fucoidan interferes with Porphyromonas gingivalis-induced aneurysm enlargement by decreasing neutrophil activation  Jean-Marc Alsac, MD, Sandrine Delbosc,

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Presentation on theme: "Fucoidan interferes with Porphyromonas gingivalis-induced aneurysm enlargement by decreasing neutrophil activation  Jean-Marc Alsac, MD, Sandrine Delbosc,"— Presentation transcript:

1 Fucoidan interferes with Porphyromonas gingivalis-induced aneurysm enlargement by decreasing neutrophil activation  Jean-Marc Alsac, MD, Sandrine Delbosc, PhD, Martin Rouer, MD, Clément Journé, MSc, Liliane Louedec, MSc, Olivier Meilhac, PhD, Jean-Baptiste Michel, MD, PhD  Journal of Vascular Surgery  Volume 57, Issue 3, Pages (March 2013) DOI: /j.jvs Copyright © 2013 Society for Vascular Surgery Terms and Conditions

2 Fig 1 Intraperitoneal fucoidan release inhibits the aneurysmal growth induced by Porphyromonas gingivalis (P. gingivalis [Pg]) injections. Maximal abdominal aortic aneurysm (AAA) diameters in each group are presented as box plots in which the median is shown. **P < .01 and ***P < .001 (one-way analysis of variance [ANOVA], Bonferroni test). C, Control; FC, fucoidan control. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2013 Society for Vascular Surgery Terms and Conditions

3 Fig 2 Circulating neutrophils were significantly increased in rats injected weekly with Porphyromonas gingivalis (P. gingivalis [Pg]). **P < .01 (one-way analysis of variance [ANOVA], Bonferroni test). C, Control; FC, fucoidan control. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2013 Society for Vascular Surgery Terms and Conditions

4 Fig 3 The increased myeloperoxydase (MPO) concentrations in plasma (A) and in abdominal aortic aneurysm (AAA)-conditioned media (B) induced by Porphyromonas gingivalis (P. gingivalis [Pg]) injections were significantly inhibited by intraperitoneal administration of fucoidan. Results are presented as box plots with median. ***P < .001 (one-way analysis of variance [ANOVA], Bonferroni test). C, Control; FC, fucoidan control. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2013 Society for Vascular Surgery Terms and Conditions

5 Fig 4 Increased cell-free DNA (cf-DNA) in plasma (A) and abdominal aortic aneurysm (AAA)-conditioned media (B) of Porphyromonas gingivalis (P. gingivalis [Pg])-injected rats was significantly inhibited by intraperitoneal fucoidan release. Results are presented as box plots with median. *P < .05, **P < .01, and ***P < .001 (one-way analysis of variance [ANOVA], Bonferroni test). C, Control; FC, fucoidan control. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2013 Society for Vascular Surgery Terms and Conditions

6 Fig 5 After fixing in paraformaldehyde, abdominal aortic aneurysm (AAA) samples of group Porphyromonas gingivalis (P. gingivalis; Pg) (A) and group fucoidan (F)+Pg (B) were embedded in paraffin and sectioned at 5 μm for histology. Slides were stained with Masson Trichrome and analyzed at ×1.25 and ×40, as presented. A, Pg injections induced neutrophil recruitment, thrombus enlargement, and inhibited healing. B, Fucoidan reduced the intraluminal thrombus (ILT) thickness in Pg-injected rats, with less neutrophil trapping, and organizing thrombus. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2013 Society for Vascular Surgery Terms and Conditions

7 Fig 6 A preservation of the elastic fiber network is induced by fucoidan on orcein-stained slides at ×40 (B), and thrombus can be seen inside the degraded internal elastic lamina in the Porphyromonas gingivalis (P. gingivalis; Pg) group (A). A qualitative indication of neutrophil increased number in abdominal aortic aneurysm (AAA) sections of the Pg group compared to the fucoidan (F)+Pg group is given by hematoxylin & eosin staining at ×20 and ×100 (C and D). P-selectin immunostaining was decreased at the luminal surface of aneurysmal aortic walls in fucoidan-treated rats, suggesting an interaction between fucoidan and P-selectin. P-selectin immunostaining of the aortic wall of AAAs of group Pg (E), and group F+Pg (F) are presented with hematoxylin nuclear counterstaining ×63. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2013 Society for Vascular Surgery Terms and Conditions

8 Fig 7 A qualitative indication of neutrophil increased number at the luminal portion of intraluminal thrombus (ILT) in the Porphyromonas gingivalis (P. gingivalis; Pg) group (A) compared to the fucoidan (F)+Pg group (B) is confirmed after 4′,6′-diamidino-2-phenylindole hydrochloride (DAPI) fluorescence at ×40. Signs of a healing process of the abdominal aortic aneurysm (AAA) treated by fucoidan are confirmed by the high density of actin stained cells into the aortic wall at ×40 of the F+Pg group (D) compared to the Pg group (C), suggesting a mesenchymal recolonization. Extranuclear DNA is visualized on DAPI fluorescence ×40, in the ILT of Pg injected AAAs (E), suggesting an enhanced neutrophil activation, whereas only nuclear DNA is seen in the fucoidan-treated aortic wall (F). Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2013 Society for Vascular Surgery Terms and Conditions

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