1. Preparation of Basic Growth Media
Key Words and Concepts
Macronutrients
Micronutrients
Fastidious organisms need lots of ____________
Solid vs. liquid media; complex vs. defined media

2. More Vocabulary Complex (undefined) vs. Synthetic (defined) media
NA vs. NB
TSA vs. TSB
Complex (undefined) vs. Synthetic (defined) media
Peptones

3. Always properly label your plates and tubes!

4. Today:

5. Making Media and Pouring Plates
Prepare 100 ml NB media in 250 ml flask
Weigh out nutrient broth powder for a 0.8% w/v solution. How much NA do you need ???
Warm the solution on the hot plate to dissolve but DO NOT boil.
After removing 5 x 4ml to tubes, how much agar do you add to make a 1.5% agar suspension of the rest ???

6. Autoclave
121 ºC
15 psi
15 minutes

9. Good luck!