1. Biotechnology and Molecular Biology
This is Jeopardy
Biotechnology and Molecular Biology
To make this game…
First think of five review categories and five questions for each category.
Add the categories to the top of the table in slide 2.
Place your questions according to the categories into the slides. For example…C1-200 is the question for Category 1 worth 200 points. C5-400 is the question for Category 5 worth 400 points.
Place a final jeopardy question in the Final Jeopardy box.
Test your slide show.
If you have any questions, please feel free to contact me at or

2. Category No. 1 Category No. 2 Category No. 3 Category No. 4 No. 5 100
200
300
400
500
To play the game.
Divide you class into teams. If you have bells or some other way of emulating ringing in your answers, knock yourself out!
The contestant chooses a category and a point value. Click on their choice and you will advance to the associated slide. Click on the slide to reveal the answer. Once the contestant has stated the question click on the slide again to reveal the question. Click BACK to return to the Categories Slide.
The first contestant from team 1 goes first then I alternate to the next team.
Final Jeopardy

3. C1-100-The answer is… Genetic Engineering
The direct manipulation of genes for practical purposes.
Genetic Engineering
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4. C1-200-The answer is… plasmid
These are small circular pieces of DNA that replicate separately from the bacterial chromosome.
plasmid
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5. C1-300-The answer is… DNA ligase
This enzyme seals the bonds between complementary restriction fragments.
DNA ligase
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6. C1-400-The answer is… Cloning vector
In gene cloning, the original plasmid is called a
Cloning vector
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7. C1-500-The answer is… YACs or Yeast Artificial Chromosomes
These eukaryotic cell parts serve as vectors to help avoid gene expression problems.
YACs or Yeast Artificial Chromosomes
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8. C2-100-The answer is… Restriction Enzymes
These cut DNA at very specific DNA sequences.
Restriction Enzymes
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9. C2-200-The answer is… Arabinose (sugar)
In the pGLO transformation experiment, the GFP protein must have _____ for the bacteria to glow.
Arabinose (sugar)
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10. C2-300-The answer is… vectors
These are used for delivery of genes into specific types of cells, for example bone marrow.
vectors
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11. C2-400-The answer is… PCR or polymerase chain reaction
This technology is used to produce many copies of a specific target segment of DNA.
PCR or polymerase chain reaction
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12. C2-500-The answer is… Gel electrophoresis
This technique uses a gel to separate nucleic acids or proteins by size.
Gel electrophoresis
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13. C3-100-The answer is… Restriction fragments
DNA that is cut with restriction enzymes yields ____.
Restriction fragments
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14. C3-200-The answer is…
What charge does DNA carry?
negative
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15. C3-300-The answer is… negative
What charge is applied to the DNA in gel electrophoresis to cause the DNA to move through the gel?
negative
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16. C3-400-The answer is… Transgenic
These animals are made by introducing genes from one species into the genome of another animal.
Transgenic
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17. C3-500-The answer is… biofuels
Environmental Cleanup – These make use of crops such as corn, soybeans, and cassava to replace fossil fuels.
biofuels
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18. C4-100-The answer is… Sticky ends
Restriction fragment ends that are staggered are called __.
Sticky ends
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19. C4-200-The answer is… The largest/longest/have the most base pairs.
In gel electrophoresis, the DNA bands that are closest to the well are ____.
The largest/longest/have the most base pairs.
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20. C4-300-The answer is… totipotent
This type of stem cell can generate any type of cell or even a whole organism.
totipotent
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21. C4-400-The answer is… Nuclear transplantation
In cloning, the nucleus of an unfertilized egg cell or zygote is replaced with the nucleus of a differentiated cell. This technique is called ___.
Nuclear transplantation
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22. C4-500-The answer is…
Genetic engineering can be used to alter the genes in food crops. These are typically called ___.
GMOs
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23. C5-100-The answer is… Blunt ends
Restriction fragments that have been cut directly across the DNA produce ___.
Blunt ends
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24. A B C D C5-200-The answer is… B, D, C, A
What is the order of the restriction fragments, from the well down, of the cut DNA? A B C D
B, D, C, A
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25. C5-300-The answer is… Gene therapy
This is the alteration of an afflicted individual’s genes.
Gene therapy
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26. C5-400-The answer is… Genetic profile
An individual’s unique DNA sequence is their _____.
Genetic profile
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27. C6-500-The answer is…
Which suspect’s DNA was found at the crime scene? 2
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28. Final Jeopardy 3 fragments that are: 3000bp 700bp 4300bp
Plasmid X is cut with restriction enzyme A. How many restriction fragments are produced? What are the sizes of the restriction fragments?
3 fragments that are:
3000bp
700bp
4300bp
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End Game