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Lina Carlén, Kazuko Sakuraba, Mona Ståhle, Fabio Sánchez 

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Presentation on theme: "Lina Carlén, Kazuko Sakuraba, Mona Ståhle, Fabio Sánchez "— Presentation transcript:

1 HLA-C Expression Pattern Is Spatially Different between Psoriasis and Eczema Skin Lesions 
Lina Carlén, Kazuko Sakuraba, Mona Ståhle, Fabio Sánchez  Journal of Investigative Dermatology  Volume 127, Issue 2, Pages (February 2007) DOI: /sj.jid Copyright © 2007 The Society for Investigative Dermatology, Inc Terms and Conditions

2 Figure 1 HLA-C expression detected by immunohistochemistry with mAb L31. (a) Tonsil, (b) adult colon epithelium, (c) fetal colon epithelium, (d) normal skin, (e) plaque psoriasis, (f) allergic contact eczema (original magnification × 20, Bar=100μM, insets: × 100, Bar=20μm) (g) LCL cells expressing HLA-Cw3 before lipopolysaccharide stimulation, and after (h) 16, (i) 48, and (j) 96hours post-stimulation (original magnification × 40, Bar=50μm). Journal of Investigative Dermatology  , DOI: ( /sj.jid ) Copyright © 2007 The Society for Investigative Dermatology, Inc Terms and Conditions

3 Figure 2 HLA-C protein expression levels in total protein extracts from psoriasis, eczema and normal skin biopsies. (a) Box plot representation of HLA-C expression according to sample group, measured by densitometry on Western blot analysis. (b) Box plot representation of HLA class I expression, measured by densitometry on Western blot analysis and detected by β2-microglobulin staining. LE=lesional eczema, LG=lesional guttate psoriasis, LP=lesional plaque psoriasis, N=normal, NLE=non-lesional eczema, NLG=non-lesional guttate psoriasis, NLP=non-lesional plaque psoriasis. Journal of Investigative Dermatology  , DOI: ( /sj.jid ) Copyright © 2007 The Society for Investigative Dermatology, Inc Terms and Conditions

4 Figure 3 Representative Western blot membrane showing total protein smears and specific HLA-C bands. (a) Ponceau staining of the skin protein extracts after transfer to a membrane. (b) Specific L31 signal. C=Control, LE=lesional eczema, LG=lesional guttate psoriasis, LP=lesional plaque psoriasis, N=normal, NLE=non-lesional eczema, NLG=non-lesional guttate psoriasis. Journal of Investigative Dermatology  , DOI: ( /sj.jid ) Copyright © 2007 The Society for Investigative Dermatology, Inc Terms and Conditions


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