1. Raman spectral study of several hemoglobins in single Red-Cell
By S.Tang
Dec. 19, 2007

2. Outline
Introduction
Our work
Future steps

3. Introduction Several hemoglobins (Hb)
The charatic peak of Raman spectrum
HbFe(II)
High spin state
HbFe(III)
HbFe(II)—O2
Low spin state
HbFe(II)—NO
1636, 1563, 1224 cm-1

4. Our work
Preparation for the samples
Analysis of the Raman Spectra

5. Preparation for the samples
Pure water
Low speed
centrifugation
Red blood cell
High speed
centrifugation Hb

6. Na2S2O4 or N2
HbFe(II) NO
HbFe(II)—O2
HbFe(III)
Na2S2O4 or N2 NO
HbFe(II)
HbFe(II)—NO

7. Analysis of the Raman Spectra
Data of Hb within a single red cell
Na2S2O4 N2
Na2S2O4+NO
K3Fe(CN)6
Natural state

8. Data of HbFe(III)

9. Future steps
To reassure the Raman Spectrum of HbFe(III) within a single red cell
To investigate whether there is certain sort of Quantum Dots (QD) that can be combined to Hb

10. Thank you!
唐爽顿首！