1. Methods Conclusion Objectives References Results
The role of BAX, BCL-2 and Caspase3 genes in Endometriotic Lesions   Roudabeh Mohammadi1, Seyed Omidreza Mousavi1, Fatemeh Khodabandehloo1, Samaneh Aghajanpour1, Fariba Ramazanali1, Ashraf Moini1, Reza Aflatoonian1* 1. Department of Endocrinology and Female infertility, Reproductive Biomedicine Center, Royan institute for ReproductiveBiomedicine, Tehran, Iran *Corresponding address :
Objectives
Methods
Not :
BAX (75 bp)
BCL (121 BP)
CASPASE3 (175 BP)
Endometriosis is characterized by the presence of endometrial cells with capacity to avoid apoptosis outside the uterus.
Apoptosis plays a fundamental role for the pathogenesis of endometriosis. The etiology and pathogenesis of endometriosis remain unclear. Apoptosis is regulated by several genes, especially those of the Bcl‐2 gene family and caspase group genes. Different expression of this genes could contribute to the survival of regurgitating endometrial cells into the peritoneal cavity and the development of endometriosis. In this study we evaluate the different expression of BCL-2, BAX and caspase 3 in endometrial tissue on endometriosis lesion.
Tissue samples involving ectopic and eutopic lesions of patients with endometriosis were collected. To compare the relative expression of BCL-2, BAX and caspase 3 Expression in endometriosis lesions the samples were divided into ectopic and eutopic groups. reverse Transcriptase PCR (RT- PCR) was performed on the prepared cDNA samples with the use of primers designed for BCL-2, BAX and caspase 3 Expression.
250 bp
Figure1 : BCL2, BAX and CASPAS3 were expressed in ectopic tissues.
Not :
BAX (75 bp)
BCL (121 BP)
CASPASE3 (175 BP)
250 bp
Figure2 : BCL2, BAX and CASPAS3 were expressed in eutopic tissues.
The Bcl-2, Bax and Caspase3 expression was observed in eutopic and ectopic samples. In eutopic tissues, caspase 3 was up-regulated.
Conclusion
Regulating Caspase-3 protein expression may be one of the ways for inducing cell apoptosis in eutopic endometrium.
References
1. Mirakhor Samani, Simin, et al. Journal of Obstetrics and Gynaecology (2018)
2. Kaya, Cihan, et al. Gynecologic and obstetric investigation (2018) 5. Ogane, Naoki, et al. Cytopathology 29.3 (2018)
3.Hu, Jin, et al.  Biochemical and biophysical research communications (2016).
Wang, Chaoqun, et al The Journal of Clinical 4.Endocrinology & Metabolism 100.7 (2015): E955-E963.
Results
Different expression of BCL-2, BAX and caspase 3 was indicated in eutopic and ectopic samples with endometriosis. In general the expression of the genes was higher in eutopic tissue