1. Loss of glomerular ESL contributes to increased glomerular albumin sieving coefficient, and modification of ESL improves glomerular albumin sieving coefficient, in old MWF rats.
Loss of glomerular ESL contributes to increased glomerular albumin sieving coefficient, and modification of ESL improves glomerular albumin sieving coefficient, in old MWF rats. (A) In healthy young MWF rats, glomerular albumin sieving coefficient values (Θalbumin) of individual glomeruli (open circles; open square represents mean ± SEM) were elevated after exposure to neuraminidase in every glomerulus (black circles; black square represents mean ± SEM). #P<0.05 versus baseline (paired t test). In contrast, higher Θalbumin values in old MWF rats (open circles; open square represents mean ± SEM) were not significantly elevated any further after neuraminidase exposure (black circles; black square represents mean ± SEM) ns, P>0.05 versus baseline (paired t test). (B) Binding of WGA lectin to the ESL (hashed bars) significantly reduced Θalbumin in old MWF rats (black bar). A similar, nonsignificant trend toward reduced Θalbumin after lectin binding was also observed in young MWF rats (open bar). *P<0.05; ns, P>0.05 versus baseline (one-way ANOVA, Bonferroni). (C) Θalbumin for individual glomeruli (gray circles, young MWF; black circles, old MWF) were significantly correlated with the percentage of glomerular capillary wall sites with ESL cover in the same glomerulus (Pearson r=−0.59; P<0.005).
Andrew H.J. Salmon et al. JASN 2012;23:
©2012 by American Society of Nephrology