1. Osteosarcoma histology.
Osteosarcoma histology. Conventional, high-grade osteosarcomas were identified in F344-Tp53 rats on long bones with frequent pulmonary metastases. (A) Bone tumor present on the distal femur with surrounding soft-tissue swelling. (B) Low magnification of a distal femur tumor with diffuse spiculated osteoid surrounded by hypercellular areas of spindle-shaped neoplastic cells. (C) Higher magnification of the boxed area in B demonstrating pleomorphic, osteoblastic spindloid neoplastic cells with production of osteoid matrix. (D) Multifocal ovoid raised mineralized lesions presenting as pulmonary metastases in fixed lung tissue. (E) Low-magnification image of perivascular metastatic neoplastic cell aggregates with foci of mineralization. (F) Higher magnification of the boxed area in E showing pleomorphic polygonal cells and spindle-shaped neoplastic cells with abundant basophilic strands of mineralization (osteoid). (B,C,E,F) Hematoxylin and eosin (H&E) stains. Scale bars: 150 µm; width of C and F represents 150 µm.
Sarah A. Hansen et al. Dis. Model. Mech. 2016;9:
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