1. Methods Objectives Results References Conclusions
ALTERED EXPRESSION OF ADAMTS-1, ADAMTS-4 AND NUCLEAR PROGESTRONE
RECEPTORS ASSOCIATED WITH IMPAIRED OOCYTE MATURITY IN PCOS PATIENTS
Tayebe Artimani¹*, Sepide Gohari taban², Iraj Amiri¹, Sara soleimani asl¹, Massoud Saidijam³, Mahnaz yavangi¹,
Nooshin Mohammadpour¹, Saeid Afshar³
¹Endometrium and Endometriosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran
² Anatomy Department, School of medicine, Hamadan University of Medical Sciences, Hamadan, Iran
³ Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
Objectives
Methods
Results
Our data revealed that a decreased expression levels of ADAMTS-1 and ADAMTS-4 in the CCs of PCOS women compared to the healthy women with normal ovarian function (p=0.02 and P=0.01 respectively )(fig1&2). Furthermore, we observed increased expression levels of ADAMTS-1 and ADAMTS-4 in the CCs of mature oocytes compared to the GV oocytes (p=0.02 and respectively) (fig3&4).In this study, we observed a strong positive correlation between the expression of progesterone receptor A with ADAMTS-1 and ADAMTS-4 (r=0.71, p=0.0001, and r=0.59, P= respectively)(fig5 & 6). The sensitivity and specificity of ADAMTS-1 were found to be 90% and 67%, respectively (an area under the ROC curve [AUC] of 0.8).
A series of proteinases, such as the matrix metalloproteinases, a Disintegrin-like and Metalloproteinase with Thrombospondin type-1 motif (ADAMTS) proteinases, have very critical roles in damage and repair of extracellular matrix (ECM) processes (remodeling). The aim of this study was to investigate changes of ADAMTS-1 and ADAMTS-4 gene expression in cumulus cells of PCOS and their association with progesterone receptors expression and oocyte Maturity.
In this study 74 infertile women years old who underwent oocyte recovery at an IVF clinic were recruited; 37 PCOS patient and 37 infertile women with normal ovulatory function as the control group. After approval of Hamadan University of Medical Sciences ethics committee and written informed consent of the patients, cumulus cells (CCs) were obtained from women undergoing oocyte retrieval and categorized according to the oocyte nuclear maturation stage: GV or MII (metaphase II stage). Total RNA was extracted and reverse transcription was performed. Gene expression of ADAMTS-1 and ADAMTS-4 was determined by quantitative real-time PCR method (q-PCR).
r= 0.59 P=
Figure 1. Expression of ADAMTS-1 in cumulus cells (CCs) of PCOS and control groups
ADAMTS-4 expression
References
PR-A expression
Figure 4. Expression of ADAMTS-4 in cumulus cells (CCs) of MII and GV oocyte
Fig. 6 Correlations between ADAMTS-4 & PRA expression in cumulus cells
Figure 2. Expression of ADAMTS-1 in cumulus cells (CCs) of MII and GV oocyte
Conclusions
r= 0.71 P=
ADAMTS-1 and ADAMTS-4 express in GV oocytes with lower levels in mature oocytes and might be an important manifestation in gonadotropin-stimulated patients which could influence oocyte maturation and can be considered as a potential diagnostic biomarker.
ADAMTS-1 expression
PR-A expression
Fig. 5 Correlations between ADAMTS-1 & PRA expression in cumulus cells
Figure 3. Expression of ADAMTS-4 in cumulus cells (CCs) of PCOS and control groups