1. The effect of omega 3-6-9 on CASA parameters of buffalo epididymal sperm in HTF cell culture medium
Abdy K1, Safar Alizadeh M 1
1Department of Clinical Sciences, Islamic Azad University, Urmia branch, Urmia, Iran;
*Corresponding author's
19th Congress on Reproductive Biomedicine & 14th Congress on Stem Cell Biology and Technology,
Objectives
It has been known sperm cells can be damaged by the process of cooling and freezing in cryopreservation and this is related to the disruption of membrane lipids resulting in mitochondrial damage and loss of integrity of acrosomal and plasma membranes. These events cause a loss and decline in motility and viability. Different rations of n-3/n-6 PUFAs improved sperm quality and increasing n-3/n-6 PUFA ratio, sperm density and motility were increased. In fact, very few studies have been conducted to examine the effects of the ratio of n-3/n-6 PUFAs on male sperm cells..
Table 1: The Effect of Omega 3,6,9 on Progressive Motility (Mean±S.E.M Class A,%)
Table 5: The Effect of Omega 3,6,9 on VAP (Mean±S.E.M, µm/s)
Methods .
Sperms were collected from 20 pairs male buffalo testicles. Collection was carried out in slaughtered animals. Sample processing was performed in a walk-in fridge (5°C) immediately after its arrival to laboratory. Then tail of epididymis was fixed between two fingers. Sperm cells were collected performing several incisions in the caudal epididymis with a surgical blade. Four levels of omega-3, 6, 9 ( mM) were added into human tubal fluid containing sperms (40×106 sperm/ml), with 10%, bovine serum albumin and were kept for 36 hours at 5 ºC. Sperm motility was examined at 1, 12, 24, 36, hours after kept in refrigerator at 5 ºC with (CASA).
Table 2: The Effect of Omega 3,6,9 on Slow Progressive Motility (Mean±S.E.M Class B,%)
Table 6 The Effect of Omega 3,6,9 on VSL (Mean±S.E.M VSL, µm/s)
Table 3: The Effect of Omega 3,6,9 on Total Motility (Mean±S.E.M ClassA+B+C,%)
References
Results
Table 7: The Effect of Omega 3,6,9 on Linearity (Mean±S.E.M,%)
Analysis of data revealed that major CASA parameters such as rapid progressive motility ( Class A %), progressive motility (Class B %), progressive motility (Class A+B+C %), velocity curvilinear (VCL µm/s), velocity average path (VAP µm/s), straight line velocity (VSL µm/s) and linearity (LIN %) were not significantly changes in omega-3,6,9 fatty acids treated groups as compared to control ( Tables 1-7).
Conclusions
These findings demonstrated that omega -3, 6, 9 at these levels had no desirable effect on motility patterns of buffalo epididymal sperm measured by CASA during storage at 5 ˚ C .
Table 4: The Effect of Omega 3,6,9 on VCL (Mean±S.E.M, µm/s)