1. Impact of systematic kinase and phosphatase k. o. on M
Impact of systematic kinase and phosphatase k.o. on M. pneumoniae phosphoproteome.
Impact of systematic kinase and phosphatase k.o. on M. pneumoniae phosphoproteome. (A) Regulated phosphosites are ordered according to whether they are (1) kinase regulated (PknB or HprK) (pink), (2) kinase and phosphatase regulated (blue) or (3) phosphatase regulated (orange). Heatmaps represent the log2 ratios of the phosphopeptides in the different strain, normalized for protein ratios. Phosphosites outside the box represent some kinase‐regulated that phosphosites could not be measured in the phosphatase k.o.: they could be assigned to either class (1) or (2). Similarly, a few phosphatase‐regulated phosphosites were not measured in both kinase k.o.. Strains and could belong to either class (2) or (3). (B) Representation of the three different phosphorylation steady states captured by the analysis. For the sites exclusively downregulated in the kinase k.o., the kinase activity largely overcomes that of the phosphatase, whereas for those also upregulated in the phosphatase k.o., kinases and phosphatase have balanced activity. Phosphosites only downregulated in the phosphatase k.o. are rare. They represent cases where the phosphatase activity largely overcomes that of the kinases. (C) Functional classification according to COG. The kinase‐regulated substrates are enriched for metabolic functions, whereas the kinase and phosphatase‐regulated substrates are enriched for Mycoplasma‐specific functions.
Vera van Noort et al. Mol Syst Biol 2012;8:571
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