1. LEUKOPOIESIS I WAYAN SUGIRITAMA http://sugiritama.blogspot.com

2. LEUKOPOIESIS “Formation and maturation of white blood cells”
Granulocytopoiesis
Agranulocytopoiesis
Monocytopoiesis
Lymphoctye maturation

3. Mature White Blood Cells

4. NEUTROPHILS

5. EOSINOPHILS

6. BASOPHILS

7. LYMPHOCYTES

8. MONOCYTES

9. BONE MARROW : The Site of Leucopoiesis
Vascular compartment
Extensive network of sinusoids .
Hemopoietic compartment
Islands of hematopoietic cells
(Hemopoietic cells in various stage of maturation )

11. HEMOPOIETIC GROWTH FACTORS Table 10-6 Gartner colour text histology page 242
Regulated the hemopoiesis
Produce by spesific cells
Acts on spesific stem cells, progenitor cells, and precursor cells
The route to deliver growth factor :
Via blood stream
Secrete near the hemopoietic cells
Direct cell-cell contact
Induced rapid mitosis or and differentiation
Most ot them are glycoproteins

12. GRANULOCYTOPOIESIS

13. GRANULOCYTOPOIESIS Formation of the granulocytes (neuthrophil,
eosinophil, and basophil)
All of Granulocyte progenitor cell derive from CFC-S
Granulocyte Progenitor :
CFU-Eo : eosinophil lineage
CFU-Ba : Basophil lineage
CFU-GM
CFU-G : Neutrophil line
CFU-M : monocyte line

15. Maturation of Granulocyte
Granulocyte maturation divide into six stages
Structural Changes :
A decreased in cell size
Condensation of nuclear chromatin
Changes in nuclear shape
An accumulation of cytoplasmic granule

16. Maturation of Granulocyte (Table 10-8 Gartner colour text histology page 242)

17. GRANULOCYTOPOIESIS
Mature granulocyte leave the bone marrow then enter the circulation→ connective tissue

18. Monocytopoiesis

20. MONOBLAST Large cell (15-25 ) Nucleus oval
Cytoplasm without granule or few azurophlic granule
Differentiate to the Promonocyte

21. PROMONOCYTE 16-18 µm in diameter Kidney shape nucleus
Cytoplasm contain azurophilic granule
Divide twice in the course of their development into monocytes

22. MONOCYTE ON BONE MARROW
Mature monocytes enter the bloodstream → circulate.
Monocyte enter the connective tissues, where they mature into macrophages.

23. LYMPHOPOIESIS

24. LYMPHOPOIESIS CFU-Ly divides form the CFU-LyB and CFU-LyT
CFU-LyB migrate to “bursa-equivalent location”, divided → B lymphocytes
CFU-LyT undergo mitosis migrate to the Thymus → T lymphocytes

26. LYMPHOBLAST

27. LYMPHOCYTE ON BONE MARROW

28. LYMPHOCYTE

29. CONCLUSION Leucocytosis is formation of WBC, occurs in Bone Marrow
Granulocyte and monocyte derive from Myeloid stem cells, whereas limfocyte from Lymphoid Stem cell
Leucocytosis involved the series of mitosis and differentiation
Leucocytosis are regulated by several growth factor and Interleukin

30. THANK YOU FOR YOUR ATTENTION
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