1. Chapter 8 Warm-Up Define the term “metabolism”.
List 3 forms of energy.
Where does the energy available for nearly all living things on earth come from?

2. An Introduction to Metabolism
Chapter 8
An Introduction to Metabolism

3. What You Need To Know: Examples of endergonic and exergonic reactions.
The key role of ATP in energy coupling.
That enzymes work by lowering the energy of activation.
The catalytic cycle of an enzyme that results in the production of a final product.
The factors that influence enzyme activity.

4. Metabolism is the totality of an organism’s chemical reactions
Manage the materials and energy resources of a cell

5. Catabolic pathways release energy by breaking down complex molecules into simpler compounds
Eg. digestive enzymes break down food → release energy
Anabolic pathways consume energy to build complex molecules from simpler ones
Eg. amino acids link to form muscle protein

6. List as many chemical reactions in a cell you can think of
Harvard Tour of a Cell
List as many types of energy that you can think of

7. Energy = capacity to do work
Kinetic energy (KE): energy associated with motion
Heat (thermal energy) is KE associated with random movement of atoms or molecules
Potential energy (PE): stored energy as a result of its position or structure
Chemical energy is PE available for release in a chemical reaction
Energy can be converted from one form to another
Eg. chemical → mechanical → electrical

9. Thermodynamics is the study of energy transformations that occur in nature
A closed system, such as liquid in a thermos, is isolated from its surroundings
In an open system, energy and matter can be transferred between the system and its surroundings
Organisms are open systems

10. Ch. 8 Warm-Up 2 What are the 1st and 2nd laws of thermodynamics?
Give the definition and an example of:
Catabolic reaction
Anabolic reaction
Is the breakdown of glucose in cellular respiration exergonic or endergonic?
Give an example of an open system and a closed system.

11. The First Law of Thermodynamics
The energy of the universe is constant
Energy can be transferred and transformed
Energy cannot be created or destroyed
Also called the principle of Conservation of Energy

12. The Second Law of Thermodynamics
Every energy transfer or transformation increases the entropy (disorder) of the universe
During every energy transfer or transformation, some energy is unusable, often lost as heat

14. Free energy: part of a system’s energy available to perform work
ΔG = change in free energy
Exergonic reaction: energy is released
Spontaneous reaction (does not mean fast, just that it happens without energy input)
ΔG < 0
Endergonic reaction: energy is required
Absorb free energy
ΔG > 0

19. Concept Check:
How does the second law of thermodynamics help explain the diffusion of a substance across a membrane?
If you place a teaspoon of sugar in the bottom of a glass of water, it will dissolve completely over time. Left longer, eventually the water will disappear and the sugar crystals will reappear. Explain these observations in terms of entropy.

20. Warmup
Catabolic reactions:
Build up or break down?
Require energy or not?
Spontaneous or not?
What does ATP stand for?
Where is ATP made?
What does ATP do for us?

21. A cell does three main kinds of work: Mechanical Transport Chemical
Energy flow into ecosystems in the form of light and exits in the form of heat.
A cell does three main kinds of work:
Mechanical
Transport
Chemical
Cells manage energy resources to do work by energy coupling: using an exergonic process to drive an endergonic one
EX: Cotransporter protein

22. ATP (adenosine triphosphate) is the cell’s main energy source in energy coupling
ATP = adenine + ribose + 3 phosphates

23. When the bonds between the phosphate groups are broken by hydrolysis → energy is released

24. How ATP Performs Work
Exergonic release of Pi is used to do the endergonic work of cell
When ATP is hydrolyzed, it becomes ADP (adenosine diphosphate)

25. Mechanical work: ATP phosphorylates motor proteins
Protein moved
Mechanical work: ATP phosphorylates motor proteins
Membrane
protein
ADP
ATP + P i P P i
Solute
Solute transported
Transport work: ATP phosphorylates transport proteins P
NH2 +
NH3
Glu + P i
Glu
Reactants: Glutamic acid
and ammonia
Product (glutamine)
made
Chemical work: ATP phosphorylates key reactants

26. Catalyst: substance that can change the rate of a reaction without being altered in the process
Enzyme = biological catalyst
Speeds up metabolic reactions by lowering the activation energy (energy needed to start reaction)

28. Substrate Specificity of Enzymes
The reactant that an enzyme acts on is called the enzyme’s substrate
The enzyme binds to its substrate, forming an enzyme-substrate complex
The active site is the region on the enzyme where the substrate binds

32. Enzyme Action: Catabolism

33. Enzyme Action: Anabolism

34. INDUCED FIT: ENZYME FITS SNUGLY AROUND SUBSTRATE -- “CLASPING HANDSHAKE”

35. An enzyme’s activity can be affected by:
temperature pH
chemicals

36. Ch. 8 Warm-Up
Draw and label the following: enzyme, active site, substrate.
Describe what is meant by the term induced fit.
What types of factors can affect an enzyme’s function?

37. Cofactors
Cofactors are nonprotein enzyme helpers such as minerals (eg. Zn, Fe, Cu)
Coenzymes are organic cofactors (eg. vitamins)
Enzyme Inhibitors
Competitive inhibitor: binds to the active site of an enzyme, competes with substrate
Noncompetitive inhibitor: binds to another part of an enzyme → enzyme changes shape → active site is nonfunctional

38. Enzyme Specificity

39. Competitive Inhibition

40. Noncompetitive Inhibition

41. Inhibition of Enzyme Activity

42. Regulation of Enzyme Activity
To regulate metabolic pathways, the cell switches on/off the genes that encode specific enzymes
Allosteric regulation: protein’s function at one site is affected by binding of a regulatory molecule to a separate site (allosteric site)
Activator – stabilizes active site
Inhibitor – stabilizes inactive form
Cooperativity – one substrate triggers shape change in other active sites → increase catalytic activity

45. Feedback Inhibition
End product of a metabolic pathway shuts down pathway by binding to the allosteric site of an enzyme
Prevent wasting chemical resources, increase efficiency of cell

46. Feedback Inhibition