1. Smooth and textured silicone surfaces of modified gel mammary prostheses cause a different impact on fibroproliferative properties of dermal fibroblasts 
Harun Seyhan, Jürgen Kopp, Justus P. Beier, Melanie Vogel, Oke Akkermann, Ulrich Kneser, Stephan Schwartz, Arndt Hartmann, Raymund E. Horch 
Journal of Plastic, Reconstructive & Aesthetic Surgery 
Volume 64, Issue 3, Pages e60-e66 (March 2011)
DOI: /j.bjps
Copyright © 2010 British Association of Plastic, Reconstructive and Aesthetic Surgeons Terms and Conditions

2. Figure 1
Representative scanning electron microscopy image of fibroblasts on smooth silicone prostheses with bar indicating 100μm in a) and 10μm in b) (energy of 200mJ (a) and 10mJ (b) per pulse, 2-Hz repetition rate, and 3.2-mm spot size).
Journal of Plastic, Reconstructive & Aesthetic Surgery  , e60-e66DOI: ( /j.bjps )
Copyright © 2010 British Association of Plastic, Reconstructive and Aesthetic Surgeons Terms and Conditions

3. Figure 2
Representative Scanning electron microscopy image of fibroblasts on textured silicone prostheses with bar indicating 30μm in a) and 10μm in b) (energy of 200mJ (a) and 10mJ (b) per pulse, 2-Hz repetition rate, and 3.2-mm spot size)
Journal of Plastic, Reconstructive & Aesthetic Surgery  , e60-e66DOI: ( /j.bjps )
Copyright © 2010 British Association of Plastic, Reconstructive and Aesthetic Surgeons Terms and Conditions

4. Figure 3
TGF β1 expression is induced in fibroblasts cultured on smooth and textured silicone surfaces. The expression of TGF β1 measured from textured surface silicone derived fibroblasts is deferred when compared to smooth surface silicone derived fibroblasts obtaining a faster value peak until the 32nd day. Subsequently fibroblasts cultured on textured silicone surfaces show a higher TGF β1 expression. 50ng RNA was reverse transcribed using Omniscript RT-PCR kit (Qiagen, Hilden, Germany). For quantitative analysis of TGF β1 mRNA, a LightCycler system from Roche (Mannheim, Germany) was used. The results were interpreted according to the manufacturers instruction. As a reference, cDNA corresponding to 200, 100, 50 or 25ng total RNA of a mixture of all samples was tested. cDNAs of different samples were analysed, each corresponding to 50ng reverse transcribed total RNA. Cycling reactions were performed as follows: 95°C, 10min–1 cycle and 95°C, 10s; 60°C, 10s; 72°C, 8s–42 cycles. The figure is representative for three independent experiments (mean values±SD of n=2).
Journal of Plastic, Reconstructive & Aesthetic Surgery  , e60-e66DOI: ( /j.bjps )
Copyright © 2010 British Association of Plastic, Reconstructive and Aesthetic Surgeons Terms and Conditions

5. Figure 4
Unstimulated fibroblasts (figure a) and expression of smooth muscle (ASM) actin after stimulation with 5ng/ml TGF-β (figure b)
Journal of Plastic, Reconstructive & Aesthetic Surgery  , e60-e66DOI: ( /j.bjps )
Copyright © 2010 British Association of Plastic, Reconstructive and Aesthetic Surgeons Terms and Conditions