1. Methods Objectives Results Conclusions References References
The effects of methamphetamine on the morphology and motility sperms in the immature male rats
Marzieh Lotfi1 M.Sc
1. M.Sc. Of Developmental Biology Department of Biology, , University of Payame Noor, Isfahan
Objectives
Results
Methods
Methamphetamine, chemicals and synthetic drug is a substance called "glass" is known, Other names it can be Hinted crystals and ice , Methamphetamine in the group is Tvanafza drugs and consumption It effects the nervous system and As a result consumer of mental and physical activity and excitement is more . Also among the substances that raises blood pressure and Causing irregular heart beat and respiration devices are stimulating . But drug abuse in society, especially among adolescents, young people who are in reproductive age has been increasing and Has become a social problem . The effects of the drug on numerous studies have been conducted on various devices adult . For Years ago, teratogenic effects and toxicity of the drug to the fetus has been of interest to researchers . Inoue et al. examines the evolution of fetal heart rats whose mothers had received prenatal methamphetamine addressed and the results showed that the drug causes fetal heart muscle cell damage and abnormal development it is . Research that has been done on the effect of the drug on the male reproductive system is limited and More focus on the testes and sperm-making is that it can work between Yamamoto noted . He continued his research on the drug showed that Desire and mating male mice with prescription of the drug decreases and to justify the finding, reported that at high doses is reduced sperm motility . In a recent study done on male genitalia, in addition to the testes, as a member of the devices special attention to the epididymis and sperm maturation in, Why so During the passage of the epididymis, sperm acquire the ability to fertilization and progressive motility the discharge conduit wall creates a perfect environment for the ultimate evolution of sperm cells . According to the dominant culture in our society, boys are more social freedom and thus they are more likely to abuse of the drug. In this study, we have examined the effects of methamphetamine on number, motility, and morphology of sperm in the epididymis immature rats (35 days old) pay.
The results of the present study shows that the average sperm motility was significantly reduced in the experimental group than the control group (P<0/001) which was dose-dependent decrease . Also according to the chart, the average sperm count was significantly reduced in the experimental group than the control group (P<0/001). According to the studies that have been done on lam stained with Eosin methylene blue, It was observed that the Sperm morphology was changed in a group of receiving methamphetamine concentrations 1,3,5mg / kg and As a result from one side decrease in the number of normal sperm (Table 1) and on the other increases abnormalities in sperm, the increase is also dose dependent (Figures 2,3 and Table 1). This process changes the morphology of sperm in the group receiving a dose 5 mg / kg is evident
Methamphetamine solution preparation: Methamphetamine were taken from the police Isfahan. The drug was solved in normal saline at concentrations specified and At certain times were injected intraperitoneally.
Sampling: Animals were randomly divided into four groups of 10: control and experimental: (1, 3, 5 mg/kg). The control group received saline and experimental groups received methamphetamine for 10 days (intraperitoneally) and they were allowed to mature. After puberty, the mice were dissected, Epididymis removed of the left testes and was cut in 2 ml of saline at 37 ° C. 20 microns of the above suspension was on the lam and the sperm motility was shot under a light microscope. The number of moving sperm were counted and Percent of the total sperm count was obtained. Then the study of sperm count, the suspension was prepared at a ratio of 1:20 with normal saline of above suspension and 10 microns were on the Neobar lam and Each of the 16 houses on the Neobar lam was counted and an average of accounted. The study of sperm morphology to 50 microns from the initial suspension was put on the lam until dry in vitro, Then the methanol for fixturing used for 5 minutes, after this period, from 5.0 percent Eosin methylene blue staining was used to and Sperm morphology was examined Under a light microscope with a magnification different. The results were analyzed by ANOVA and Duncan's test and level (P<0/05) was considered significant.
Conclusions
Frequent consumption of methamphetamine even in low doses decrease the number and mobility of mature and normal sperms and also increases abnormalities in sperm morphology, which all show that the consumption of methamphetamine is likely to reduce fertility or sterility in males.
References
1. O'Malley P. Ecstasy for intimacy: Potentially fatal choices for adolescents and young adults: update for the clinical nurse specialist. Clin Nurse Spec 2005; 19(2): 63-4.
2. Yamamoto Y, Yamamoko K., Hayase T. Effect of methamphetamine on male mice fertility. J Obstet
Gynecol Res 1999; 25(5):
3. Malihe Heidari-Rarani, Ali Noori, Arash Ghodousi. Effects of Methamphetamin on Pituitary Gonadal Axis and Spermatogenesis in Matur Male Rats, Zahedan J Res Med Sci 2014 Dec; 16(12): 35
4. Lohiya NK, Mishra PK, Pathak N, Manivannan B, Bhande SS, Panneerdoss S, Sriram S. Efficacy trial on the purified compounds of the seeds of Carica papaya for male contraception in albino rat. Repord Toxicol 2005; 20(1):
5. Srikanth V, Malini T, Arunakaran J, Govindarajulu P, Balasubramanian K. Effects of ethanol treatment on epididymal secretory products and sperm maturation in albino rats. J pharmacol Exp Ther 1999; 288(2):
Table 1. Average morphology of sperms in the treatment and control groups
Twisted flagella
Disruption of the head connection
Disruption of flagella
Normal head without flagella
Normal flagella without head
Nrmal Sperm
Density (mg/kgw)
0.57±0.535
0.43±0.535
2.57±1.134
1.57±0.976
1.71±0.488
47.71±5.407
Controls
1.14±0.378
1.71±0.756
2.43±0.787
2.71±0.488
2.43±1.272
15.86±3.716 1
2.57±1.397
4.86±0.690
3.29±1.113
15.71±3.039 3
2.71±0.951
10±2.160
6.43±1.272
3.43±0.787
15.29±3.773 5
0.05>P ، 3.200=F
0.001>P ، 8.308=F
0.001>P ، =F
0.001>P، =F
0.01>P ، 4.821=F
0.001>P ، =F
The significance level
References
Fig1. Normal Sperm - Made with blue eosin methylene - Magnification × 40
Fig2. Normal flagella without head & Normal head without flagella - Made with blue eosin methylene - Magnification × 40
Fig3. disruption of flagella - Made with blue eosin methylene - Magnification × 40
Fig 1. A comparison of the changes in the average percent of sperm mobility, between the treatment group and the control group with segrated concentration. *** Showing Significant reduction (p<0.001) in the treatment group in comparison to the control group
Fig 2. A comparison of the changes in the average number of sperms between the treatment groups and the control group with segrated concentration. *** Showing significant reduction (P<0.001) in the treatment group in comparison to the control group.
Sperm mobility in control group
Sperm mobility in experimental group
(5 mg / kg)