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Ruben D. Motrich, M. Sc. , Andres A. Ponce, Ph. D. , Virginia E

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Presentation on theme: "Ruben D. Motrich, M. Sc. , Andres A. Ponce, Ph. D. , Virginia E"— Presentation transcript:

1 Effect of tamoxifen treatment on the semen quality and fertility of the male rat 
Ruben D. Motrich, M.Sc., Andres A. Ponce, Ph.D., Virginia E. Rivero, Ph.D.  Fertility and Sterility  Volume 88, Issue 2, Pages (August 2007) DOI: /j.fertnstert Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions

2 Figure 1 Sperm quality analysis in seminal sperm. Analysis of copulatory plugs (A), sperm concentration (B), sperm motility (C), sperm viability (D), and HOS test (E) was carried out in semen samples obtained by electroejaculation of tamoxifen-treated male rats (group T). Different parameters of spermatozoa were determined immediately after sperm obtainment by evaluation following standard methods. In all cases samples were processed individually, and five animals per group were analyzed. ∗P<.05 versus control group (group C). Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions

3 Figure 2 Epididymal sperm quality analysis. Analysis of sperm concentration (A), sperm motility (B), sperm viability (C), and HOS test (D) were carried out in epididymal sperm samples of tamoxifen-treated male rats (group T). Different parameters of spermatozoa were determined immediately after epididymal sperm obtainment by evaluation following standard methods. In all cases samples were processed individually, and five animals per group were analyzed. ∗P<.05 versus control group (group C). Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions

4 Figure 3 Testicular histologic analysis: tamoxifen-induced sloughing of the seminiferous epithelium. Light micrographs of control animal (group C; A: ×100; B: ×600) and tamoxifen-treated animal testes morphology (group T; C: ×200; D: ×600). A significant increase in the number of partial sloughing tubules was observed in testes from tamoxifen-treated animals when compared with controls. In all cases samples were processed individually, and five animals per group were analyzed. From each animal, six sections cut at 10 μm apart were screened. One hundred seminiferous tubules were counted, and sloughed tubules were classified. ∗P<.01 versus control group. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions


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