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Sup35 Tda1 Pgk1 BY4743 Brightfield Chemiluminiscence.

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Presentation on theme: "Sup35 Tda1 Pgk1 BY4743 Brightfield Chemiluminiscence."— Presentation transcript:

1 Sup35 Tda1 Pgk1 BY4743 Brightfield Chemiluminiscence

2 TAP tag adds approximately 20 kDa of additional peptide sequence
Gel 2 (Gr 6, 5) Sup35 Tda1 Pgk1 TAP tag adds approximately 20 kDa of additional peptide sequence BY4743 245 Sup35+TAP=97,549 kDa Tda1+TAP=87,217 kDa Pgk1+TAP= 65,739 kDa 190 135 100 80 58 46 32 25 Chemiluminescence inverted and brightfield merged

3 Pgk1 Sup35 Tda1 Sup35 Tda1 BY4743 Brightfield Chemiluminiscence

4 Chemiluminiscence inverted and brightfield merged
Gel 1 (Group 1, 2, 3) Sup35 Tda1 Pgk1 BY4743 135 135 100 100 80 80 58 58 46 46 32 32 25 25 Chemiluminiscence inverted and brightfield merged

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