Presentation is loading. Please wait.

Presentation is loading. Please wait.

Chronic treatment of hydroxytryptamine type 2a receptor antagonist sarpogrelate hydrochloride modulates the vasoreactivity of serotonin in experimental.

Published bySusanti Sudirman Modified over 6 years ago

Similar presentations

Presentation on theme: "Chronic treatment of hydroxytryptamine type 2a receptor antagonist sarpogrelate hydrochloride modulates the vasoreactivity of serotonin in experimental."— Presentation transcript:

1 Chronic treatment of hydroxytryptamine type 2a receptor antagonist sarpogrelate hydrochloride modulates the vasoreactivity of serotonin in experimental rabbit vein grafts  Akio Kodama, MD, PhD, Kimihiro Komori, MD, PhD, Junko Kajikuri, PhD, Takeo Itoh, PhD  Journal of Vascular Surgery  Volume 50, Issue 3, Pages (September 2009) DOI: /j.jvs Copyright © 2009 Society for Vascular Surgery Terms and Conditions

2 Fig 1 Immunostaining of 5-hydroxytryptamine (5-HT) subtype receptors in vein grafts from control rabbits (control group) and sarpogrelate hydrochloride (SH)-treated rabbits (SH-treated group). A, Immunofluorescence staining against 5-HT1B receptors in control group (Aa) and SH-treated group (Ab). Arrow heads indicate the intima and medial regions. Inserted panel: Negative control data was obtained by application of second antibody only (ie, without application of primary antibody against each subtype of serotonin receptors) to show background staining. Similar observations were made in other three different sections from three other rabbits. Ac, Shows summary of data obtained from four samples from four different animals in each group. B, Immunofluorescence staining against 5-HT2A receptors in control group (Ba) and SH-treated group (Bb). Arrow heads indicate the intima and medial regions. Inserted panel: Negative control data was obtained by application of second antibody only (ie, without application of primary antibody against each subtype of serotonin receptors) to show background staining. Bc, Shows summary of data from four samples from four different rabbits in each group. Data are shown as mean ± SEM. *P < .05 vs control group (unpaired t test). Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2009 Society for Vascular Surgery Terms and Conditions

3 Fig 2 (Protocol 1) The 5-hydroxytryptamine (5-HT)-induced contraction in endothelium-intact vein grafts from control rabbits (control group) and sarpogrelate hydrochloride (SH)-treated rabbits (SH-treated group). Effects of in vitro applied the 5-HT1B receptor antagonist GR55562 alone and together with the 5-HT2A receptor antagonist SH in control group (A) or SH-treated group (B). The maximum amplitude of contraction induced by 5-HT (1 μM) was defined as a relative tension of 1.0 for each strip. Mean of data from six strips from six other animals, with SEM shown by vertical line. *P < .05, **P < .01 vs none in each group, †P < .05, ††P < .01 vs in the presence of GR55562 alone (two-way repeated-measures analysis of variance (ANOVA) followed by Scheffé's post hoc F test). Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2009 Society for Vascular Surgery Terms and Conditions

4 Fig 3 Effects of l-NG-nitroarginine (l-NNA) on 5-HT-induced contraction in endothelium-intact vein grafts from control rabbits (control group, A) and sarpogrelate hydrochloride (SH)-treated rabbits (SH-treated group, B). l-NNA(−), in the absence l-NNA; l-NNA(+), in presence of l-NNA; l-NNA(+) + GR55562, in the presence of l-NNA and GR The maximum amplitude of contraction induced by 5-hydroxytryptamine (5-HT) (1 μM) was defined as a relative tension of 1.0 for each strip. Mean of data from six strips from six other animals, with SEM shown by vertical line. **P < .01 vs in the absence of l-NNA, †P < .05, vs in the presence of l-NNA and GR55562 (two-way repeated-measures analysis of variance [ANOVA] followed by Scheffé's post hoc F test). Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2009 Society for Vascular Surgery Terms and Conditions

5 Fig 4 (Protocol 2) Effect of l-NNA on 5-hydroxytryptamine (5-HT)-induced contraction in vein grafts from control rabbits (control group) and sarpogrelate hydrochloride (SH)-treated rabbits (SH-treated group) in the presence of sarpogrelate and prostaglandin F2α (PGF2α). A, An actual trace in an endothelium-intact vein graft from SH-treated group. The 5-HT was cumulatively applied from low to high concentration during the contraction induced by PGF2α in the presence of SH. Summary of the effect of l-NNA in endothelium-intact vein grafts from control rabbits (B), endothelium-intact vein grafts from SH-treated group (C) and endothelium-denuded vein grafts from SH-treated group (D). l-NNA (−) and (+), in the absence and presence of l-NNA, respectively. The maximum amplitude of contraction induced by 5-HT (1 μM) in the absence or presence of l-NNA was defined as a relative tension of 1.0 for each strip. Mean of data from five strips, with SEM shown by vertical line. *P < .05 vs in the absence of l-NNA (two-way repeated-measures analysis of variance [ANOVA] followed by Scheffé's post hoc F test). Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2009 Society for Vascular Surgery Terms and Conditions

6 Fig 5 Effect of GR55562 on 5-hydroxytryptamine (5-HT)-induced contraction in endothelium-intact vein grafts from control rabbits (control group, A) and sarpogrelate hydrochloride (SH)-treated rabbits (SH-treated group, B). l-NNA and SH were present throughout the experiments. The maximum amplitude of contraction induced by 5-HT (1 μM) in the absence of GR55562 was defined as a relative tension of 1.0 for each strip. Mean of data from five strips, with SEM shown by the vertical line. *P < .05, **P < .01 vs in the absence of GR55562 (two-way repeated-measures analysis of variance [ANOVA] followed by Scheffé's post hoc F test). Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2009 Society for Vascular Surgery Terms and Conditions

Download ppt "Chronic treatment of hydroxytryptamine type 2a receptor antagonist sarpogrelate hydrochloride modulates the vasoreactivity of serotonin in experimental."

Similar presentations

Professionalism and the work-life balance Thomas S. Huber, MD, PhD Journal of Vascular Surgery Volume 60, Issue 4, Pages 1072-1082 (October 2014) DOI:

Professionalism and the work-life balance Thomas S. Huber, MD, PhD Journal of Vascular Surgery Volume 60, Issue 4, Pages (October 2014) DOI:
Lucy Wales, FRCS, MD, Martin Gosling, PhD, Graham W

Lucy Wales, FRCS, MD, Martin Gosling, PhD, Graham W
Propionyl-L-carnitine dilates human subcutaneous arteries through an endothelium- dependent mechanism  Marilyn J. Cipolla, PhD, Alexander Nicoloff, MD,

Propionyl-L-carnitine dilates human subcutaneous arteries through an endothelium- dependent mechanism  Marilyn J. Cipolla, PhD, Alexander Nicoloff, MD,
Without prejudice Journal of Vascular Surgery

Without prejudice Journal of Vascular Surgery
Dipeptidyl peptidase 4 inhibitor reduces intimal hyperplasia in rabbit autologous jugular vein graft under poor distal runoff  Akio Koyama, MD, Kimihiro.

Dipeptidyl peptidase 4 inhibitor reduces intimal hyperplasia in rabbit autologous jugular vein graft under poor distal runoff  Akio Koyama, MD, Kimihiro.
Inflammatory responses involving tumor necrosis factor receptor-associated factor 6 contribute to in-stent lesion formation in a stent implantation model.

Inflammatory responses involving tumor necrosis factor receptor-associated factor 6 contribute to in-stent lesion formation in a stent implantation model.
Lloyd M. Taylor, M.D., Edward S. Phinney, M.D., John M. Porter, M.D. 

Lloyd M. Taylor, M.D., Edward S. Phinney, M.D., John M. Porter, M.D. 
Nathan R. Tykocki, PhD, BinXi Wu, BS, William F

Nathan R. Tykocki, PhD, BinXi Wu, BS, William F
Celiprolol reduces the intimal thickening of autogenous vein grafts via an enhancement of nitric oxide function through an inhibition of superoxide production 

Celiprolol reduces the intimal thickening of autogenous vein grafts via an enhancement of nitric oxide function through an inhibition of superoxide production 
Ezetimibe reduces intimal hyperplasia in rabbit jugular vein graft

Ezetimibe reduces intimal hyperplasia in rabbit jugular vein graft
Tumor necrosis factor-α and the early vein graft

Tumor necrosis factor-α and the early vein graft
Altered coronary microvascular serotonin receptor expression after coronary artery bypass grafting with cardiopulmonary bypass  Michael P. Robich, MD,

Altered coronary microvascular serotonin receptor expression after coronary artery bypass grafting with cardiopulmonary bypass  Michael P. Robich, MD,
Wei Zhou, MD, Hong Chai, MD, PhD, Peter H. Lin, MD, Alan B

Wei Zhou, MD, Hong Chai, MD, PhD, Peter H. Lin, MD, Alan B
Endothelium-Dependent Vasoconstriction in Isolated Vessel Grafts: A Novel Mechanism of Vasospasm?  Markus Hoenicka, PhD, Andreas Keyser, MD, Leopold Rupprecht,

Endothelium-Dependent Vasoconstriction in Isolated Vessel Grafts: A Novel Mechanism of Vasospasm?  Markus Hoenicka, PhD, Andreas Keyser, MD, Leopold Rupprecht,
James C. Harms, MD, Sunil Rangarajan, MD, Carlton J

James C. Harms, MD, Sunil Rangarajan, MD, Carlton J
Peter J. Fahner, MD, Mirza M. Idu, MD, PhD, Thomas M

Peter J. Fahner, MD, Mirza M. Idu, MD, PhD, Thomas M
Suppression of Graft Spasm by the Particulate Guanylyl Cyclase Activator in Coronary Bypass Surgery  Takeshi Kinoshita, MD, PhD, Masashi Tawa, PhD, Tomoaki.

Suppression of Graft Spasm by the Particulate Guanylyl Cyclase Activator in Coronary Bypass Surgery  Takeshi Kinoshita, MD, PhD, Masashi Tawa, PhD, Tomoaki.
Substance P Enhances the Production of Interferon-induced Protein of 10 kDa by Human Keratinocytes in Synergy with Interferon-γ  Naoko Kanda, Shinichi.

Substance P Enhances the Production of Interferon-induced Protein of 10 kDa by Human Keratinocytes in Synergy with Interferon-γ  Naoko Kanda, Shinichi.
Dipeptidyl peptidase 4 inhibitor reduces intimal hyperplasia in rabbit autologous jugular vein graft under poor distal runoff  Akio Koyama, MD, Kimihiro.

Dipeptidyl peptidase 4 inhibitor reduces intimal hyperplasia in rabbit autologous jugular vein graft under poor distal runoff  Akio Koyama, MD, Kimihiro.
Increased connexin43 expression in human saphenous veins in culture is associated with intimal hyperplasia  Sébastien Déglise, MD, David Martin, MS, Hervé.

Increased connexin43 expression in human saphenous veins in culture is associated with intimal hyperplasia  Sébastien Déglise, MD, David Martin, MS, Hervé.

Similar presentations

Ads by Google