1. Severe deficiency of switched memory B cells (CD27+IgM−IgD−) in subgroups of patients with common variable immunodeficiency: a new approach to classify a heterogeneous disease
by Klaus Warnatz, Axel Denz, Ruth Dräger, Moritz Braun, Christoph Groth, Guido Wolff-Vorbeck, Hermann Eibel, Michael Schlesier, and Hans Hartmut Peter
Blood
Volume 99(5):
March 1, 2002
©2002 by American Society of Hematology

2. Phenotypes of peripheral B cells of CVID patients
Phenotypes of peripheral B cells of CVID patients.PBMCs were stained for the expression of CD19, CD27, IgM, and IgD and analyzed by FACS. Analysis was performed by gating on CD19+cells, and dead cells were excluded by forward/side scatter gating.
Phenotypes of peripheral B cells of CVID patients.PBMCs were stained for the expression of CD19, CD27, IgM, and IgD and analyzed by FACS. Analysis was performed by gating on CD19+cells, and dead cells were excluded by forward/side scatter gating. Representative examples of each CVID subgroup (A-C) according to the classification by Bryant et al9 as well as one healthy control (HD) are given. Indicated values represent percentages of gated CD19+ B cells. See “Patients, materials, and methods.”
Klaus Warnatz et al. Blood 2002;99:
©2002 by American Society of Hematology

3. Profound decrease of switched memory B cells in CVID patients of subgroups A and B.PBMCs were stained for the expression of CD19, CD27, IgM, and IgD, as shown in Figure 1.
Profound decrease of switched memory B cells in CVID patients of subgroups A and B.PBMCs were stained for the expression of CD19, CD27, IgM, and IgD, as shown in Figure 1. CVID patients are assigned to groups A-C according to the classification by Bryant et al9 and are compared with healthy controls (HD). Rhombus symbols represent the percentage of switched memory B cells (CD27+IgM−IgD−) for each patient or healthy donor. Statistical analysis was performed by the Student t test, and P values are given.
Klaus Warnatz et al. Blood 2002;99:
©2002 by American Society of Hematology

4. Good correlation between percentage of switched memory B cells in peripheral blood and IgG synthesis in vitro.The percentage of switched memory B cells (CD27+IgM−IgD−) in the peripheral blood and IgG production after SAC and IL-2 stimulation in vitro show a...
Good correlation between percentage of switched memory B cells in peripheral blood and IgG synthesis in vitro.The percentage of switched memory B cells (CD27+IgM−IgD−) in the peripheral blood and IgG production after SAC and IL-2 stimulation in vitro show a significant correlation (r = 0.81) when analyzed by linear regression. Healthy controls as well as CVID patients are included in this plot.
Klaus Warnatz et al. Blood 2002;99:
©2002 by American Society of Hematology

5. Increase of CD21− B cells in CVID patients of group Ia
Increase of CD21− B cells in CVID patients of group Ia.PBMCs were stained for the expression of CD19, CD21, IgM, and IgD and analyzed by FACS. Analysis was performed by gating on CD19+B cells, and dead cells were excluded by forward/side scatter gating.
Increase of CD21− B cells in CVID patients of group Ia.PBMCs were stained for the expression of CD19, CD21, IgM, and IgD and analyzed by FACS. Analysis was performed by gating on CD19+B cells, and dead cells were excluded by forward/side scatter gating. Representative FACS dot blots of the new CVID subgroups Ia, Ib, II, and one healthy control (HD) are shown. Indicated values represent percentages of gated CD19+ B cells. See “Patients, materials, and methods.”
Klaus Warnatz et al. Blood 2002;99:
©2002 by American Society of Hematology