1. Virtual Endoscopy Based on 3-Dimensional Reconstruction of Histopathology Features of Endoscopic Resection Specimens 
Rüdiger Schmitz, Jenny Krause, Till Krech, Thomas Rösch 
Gastroenterology 
Volume 154, Issue 5, Pages e4 (April 2018)
DOI: /j.gastro
Copyright © 2018 AGA Institute Terms and Conditions

2. Figure 1
The 3-dimenaional reconstructed model of the specimen pathologies (A) provides a “pathology map” (B) of the original specimen (C; yellow, metaplasia; orange, low-grade intraepithelial neoplasia; red, high-grade intraepithelial neoplasia; light/dark gray, squamous/cardia epithelium).
Gastroenterology  , e4DOI: ( /j.gastro )
Copyright © 2018 AGA Institute Terms and Conditions

3. Figure 2
Virtual and augmented virtual endoscopy. The asterisks, arrows, and crosses mark corresponding structures in the original (A), virtual (B), and augmented virtual endoscopy views (C; yellow, metaplasia; orange, low-grade intraepithelial neoplasia; red, high-grade intraepithelial neoplasia).
Gastroenterology  , e4DOI: ( /j.gastro )
Copyright © 2018 AGA Institute Terms and Conditions

4. Supplemental Figure 1
Prereconstruction workflow includes endoscopic resection of the lesion (A), followed by histopathologic processing (B) and annotations (C, D). The latter comprises manual annotation of the epithelial and pathology types (C)—low-grade dysplasia (yellow) and squamous epithelium (gray) in this example—and the line of the deepest extent of the epithelium (D, green line and crosses). The photographs (A, B) and their analogues shown in the main text (Figures 1C and 2A with the background being removed in the former) have been edited for optimized brightness, saturation, and sharpness. LGIN, low-grade intraepithelial neoplasia.
Gastroenterology  , e4DOI: ( /j.gastro )
Copyright © 2018 AGA Institute Terms and Conditions

5. Supplemental Figure 2
The deformed original tissue section (A, top) is transformed to a globally straight form (A, bottom) without impairing its local features (C, D) by use of anatomic landmarks. A, The line of deepest extent of the epithelium (green line), the border to the former esophagus lumen (blue line), and the resection edge (red line) serve as anatomic landmarks for computation of the normalization transformation (black arrows). The positions in both the original and the normalized section are parameterized with respect to the line of the deepest epithelial extent by the length s along that line and the distance d from it, which simplifies the computation of the vector field that describes the normalization transformation. B, Detailed view of a region from the original tissue section. C, The corresponding region inside the normalized tissue section.
Gastroenterology  , e4DOI: ( /j.gastro )
Copyright © 2018 AGA Institute Terms and Conditions

6. Supplemental Figure 3
Virtual (A) and augmented virtual (B) endoscopy of the 3-dimensional reconstructed endoscopic submucosal dissection specimen. The lesion is situated directly at the Z-line, the transition from squamous (beige and light gray) to cardia epithelium (red and dark gray). The direction of view is in gastral direction. The augmented virtual endoscopy view (B) reveals the structure of the lesion with intestinal metaplasia (yellow), low-grade intraepithelial neoplasia (LGIN, orange) and high-grade intraepithelial neoplasia (HGIN, red).
Gastroenterology  , e4DOI: ( /j.gastro )
Copyright © 2018 AGA Institute Terms and Conditions