1. Detection of CD38+IgG1+ memory B cells adjacent to contracted GCs
Detection of CD38+IgG1+ memory B cells adjacent to contracted GCs. (A) B6 mice were immunized with alum-precipitated NP-CGG.
Detection of CD38+IgG1+ memory B cells adjacent to contracted GCs. (A) B6 mice were immunized with alum-precipitated NP-CGG. After 30 and 60 days, spleens were harvested and subjected to flow cytometric (Upper) and immunohistological (Lower) analyses. B220+ gated populations are shown in the upper panels. A fluorescence-activated cell sorting (FACS) profile of an unimmunized B6 mouse (day 0) is also shown. White arrows in the first and second rows of the second section indicate cells stained with both anti-CD38 and -IgG1 Abs, and in the third row indicate cells that are IgG1+λ+. [Scale bars, 150 μm (Left), 50 μm (Center).] (B) Quantification of the absolute number of memory (CD38+IgG1+) and GC (CD38−IgG1+) B cells by flow cytometric (Left) and immunohistological (Right) analyses.
Yuichi Aiba et al. PNAS 2010;107:27:
©2010 by National Academy of Sciences