1. Development of a Device for Neurochemical Sample Collection from Freely Moving Monkeys
Team Members:
Kara Barnhart
Angwei Law
Paul Schildgen
Grant Smith
Client:
Dr. Ei Terasawa, Dept. of Pediatrics
Advisor:
Professor William Murphy, Dept. of BME

2. Outline Client Research Experimental Setup Free Monkeys from Chairing
Design Requirements
Solution Ideas
Construction & Testing
Ethical Considerations

3. Background-Research Control of growth and development
Brain’s role in triggering puberty
Measures neuropeptides in hypothalamus for neuroendocrine studies
Rhesus monkeys as model system for humans
Associated Press. “Scientists Map Genome of Rhesus Monkey” 13 Apr 2007.
4 Mar <

4. Background-Experimental Setup
Metal ring implanted into skull
Microdrive unit attached
Monkey transferred to chair
Microdialysis probe inserted
Tubing attached to fraction collector and pump
Monkey confined to chair for 12 hours
Forst SI, et al., Microdialysis methods for in vivo neropeptide measurement in the Stalk-median eminence in the Rhesus monkey, J Neurosci Methods (2007), doi: /j.jneumeth
SAEN. The Mary T. and Frank L. Hoffman Family Foundation. 11 Feb Mar <

5. Problem Statement
Construct protective device for microdialysis apparatus
Used during cranial experiments on monkey
Free the monkey from the constraints of chairing
Provide a more comfortable and realistic environment for the monkey
Monkey attached to tether in cage instead of chair

6. PDS
Client: Design a device to protect the microdrive unit to free monkey from chairing
Safe for monkey
Weight: Under 0.5 kg
Sterile
Safe from monkey
Force: Withstand 100 N
Stable
Detachable

7. Design Alternative-Jacket
Monkey wears jacket
Metal rod attached
Metal ring around head
Straps from metal ring around chin and neck
PVC pipe covering device

8. Design Alternative-Helmet
Plastic helmet with straps
Hole at center of helmet
PVC pipe attached to helmet

9. Design Alternative-Cushion Cylinder
3 piece aluminum casing
Soft molded polymer core
Straps around chin and neck attached to casing
Secured with an Allen wrench

10. Design Matrix 15 18 7 10 25 3 8 5 6 53 70 67 Jacket with rod and ring
Jacket with rod and ring
Cushion
Cylinder
Helmet
Security from Monkey
(30) 15 18
Security from Slip
(20) 7 10
Ability to Withstand Force 25
Ease of Use
(10) 3 8
Ease of Construction 5 6
Safety
Total 53 70 67

11. Future Work Figure out exact dimensions Order materials and parts
Construct prototype
Test device

12. Ethical Considerations
Research abides by strict federal regulations
Monkeys under constant supervision and care
Monkeys are not harmed or ill-treated during the experiment

13. Credits
Ei Terasawa
Kim Keen
Professor Murphy
Primate Center Staff

14. Questions?