1. Macromolecule Testing
Subtitle

2. What are our samples? Water Based on test Glucose= Sugar
Starch = corn starch
Protein = Albumin
Lipid = Cooking oil
Stomach Contents

3. Carbohydrates (Glucose)
Benedict’s Test
In a test tube, add 10 drops of your sample
Add 5 drops of Benedict’s solution
Place the tubes in a hot water bath for 5 minutes
Use test tube clamps and hot pads
Record detailed observations
Indicator = color change

4. Carbohydrates (Starch)
Iodine Test
In a well tray, add 3-4 drops of your sample
Add 2 drops of Iodine solution to the well
Record detailed observations
Indicator = color change

5. Proteins Biuret’s Test In a well tray, add 3-4 drops of your sample
Add 2-3 drops of Biuret’s solution to the well
Record detailed observations
Indicator = color change

6. Lipids
Drop Test
Draw three separate circles on a piece of paper, label 1, 2, 3
Place a drop of the sample on its respective circle
1=water, 2=cooking oil, 3= stomach
Wait to dry for 5 minutes, and record observation

7. Lipids Sudan III Test In a well tray, add 3-4 drops of your sample
Add 2 drops of Sudan III solution
Record observations in detail
Indicator = color change, layering

8. What to wear
Close-toed shoes
Goggles
Apron
Oven mitts (if needed)