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Persistent carbapenem-resistant Klebsiella pneumoniae: a Trojan horse

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1 Persistent carbapenem-resistant Klebsiella pneumoniae: a Trojan horse
Zhi-Qiang Yang, Yong-Lu Huang, Hong-Wei Zhou, Rong Zhang, Kui Zhu  The Lancet Infectious Diseases  Volume 18, Issue 1, Pages (January 2018) DOI: /S (17) Copyright © 2018 Elsevier Ltd Terms and Conditions

2 Figure Representative fluorescence images of cells infected with CRKP
(A) Intracellular CRKP 7 in intestinal epithelial cells 6 (IEC-6 cells), with a multiplicity of infection of 100. (B) Three-dimensional image of (A). CRKP are located in the cytosol, rather than attaching to the cellular membrane. Images were obtained using Leica TCS SP8 confocal laser scanning microscopy. Bacteria were stained with anti-K pneumoniae primary antibody (Abcam, Cambridge, UK) followed by Alexa Fluor 488 conjugated secondary antibody (green; Beyotime Institute of Biotechnology, Haimen, China), and F-actin with rhodamine phalloidin (red; Invitrogen, CA, USA). Nuclei were stained with 4',6-diamidino-2-phenylindole (blue; Beyotime Institute of Biotechnology). CRKP= carbapenem-resistant Klebsiella pneumoniae. The Lancet Infectious Diseases  , 22-23DOI: ( /S (17) ) Copyright © 2018 Elsevier Ltd Terms and Conditions


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