Presentation is loading. Please wait.

Presentation is loading. Please wait.

Immunoguided Laser Assisted Microdissection Techniques for DNA Methylation Analysis of Archival Tissue Specimens  Franziska C. Eberle, Jeffrey C. Hanson,

Published byMadeline Ball Modified over 6 years ago

Similar presentations

Presentation on theme: "Immunoguided Laser Assisted Microdissection Techniques for DNA Methylation Analysis of Archival Tissue Specimens  Franziska C. Eberle, Jeffrey C. Hanson,"— Presentation transcript:

1 Immunoguided Laser Assisted Microdissection Techniques for DNA Methylation Analysis of Archival Tissue Specimens  Franziska C. Eberle, Jeffrey C. Hanson, J. Keith Killian, Lai Wei, Kris Ylaya, Stephen M. Hewitt, Elaine S. Jaffe, Michael R. Emmert- Buck, Jaime Rodriguez-Canales  The Journal of Molecular Diagnostics  Volume 12, Issue 4, Pages (July 2010) DOI: /jmoldx Copyright © 2010 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

2 Figure 1 Technical steps for immunohistochemistry of formalin-fixed paraffin-embedded tissue sections on membrane slides for subsequent laser-assisted microdissection and DNA methylation profiling. The Journal of Molecular Diagnostics  , DOI: ( /jmoldx ) Copyright © 2010 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

3 Figure 2 CD30 staining of Hodgkin-Reed-Sternberg cells in a Hodgkin's lymphoma on a regular glass slide (A) and on a polyethylene naphthalate membrane slide (B). Both sections of the FFPE sample, either on glass slide or membrane slide, are shown without hematoxylin counterstaining and without cover slide; original magnification, ×40. The Journal of Molecular Diagnostics  , DOI: ( /jmoldx ) Copyright © 2010 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

4 Figure 3 CD30+ Hodgkin-Reed-Sternberg cells in a Hodgkin's lymphoma before microdissection (A), selected for microdissection (B), and after microdissection (C) using the Leica LMD 6000; original magnification, ×63. The Journal of Molecular Diagnostics  , DOI: ( /jmoldx ) Copyright © 2010 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

5 Figure 4 PCR products after PCR amplification of the β-globin gene using three different sets of primers. A: Detection of 152-bp amplicon. B: Detection of 268-bp amplicon. C: Detection of 676-bp amplicon. P indicates positive control; H, PCR of microdissected tumor cells stained with H&E; M, molecular weight marker; I, PCR of microdissected tumor cells stained with immunohistochemistry; N, negative control. PCR of controls, H, and I were performed as duplicates. The Journal of Molecular Diagnostics  , DOI: ( /jmoldx ) Copyright © 2010 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

6 Figure 5 Illumina GoldenGate Methylation array of five individual cases. Each plot shows the correlation of target CpG methylation of DNA isolated from H&E-stained tumor cells (x axis) compared with DNA isolated from immunohistochemically stained tumor cells (y axis). A minimum correlation of r = 0.94 and a maximum correlation of r = 0.98 was observed. The Journal of Molecular Diagnostics  , DOI: ( /jmoldx ) Copyright © 2010 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Download ppt "Immunoguided Laser Assisted Microdissection Techniques for DNA Methylation Analysis of Archival Tissue Specimens  Franziska C. Eberle, Jeffrey C. Hanson,"

Similar presentations

Development of a Novel One-Tube Isothermal Reverse Transcription Thermophilic Helicase-Dependent Amplification Platform for Rapid RNA Detection James Goldmeyer,

Development of a Novel One-Tube Isothermal Reverse Transcription Thermophilic Helicase-Dependent Amplification Platform for Rapid RNA Detection James Goldmeyer,
CyclinD1/CyclinD3 Ratio by Real-Time PCR Improves Specificity for the Diagnosis of Mantle Cell Lymphoma Carol D. Jones, Katherine H. Darnell, Roger A.

CyclinD1/CyclinD3 Ratio by Real-Time PCR Improves Specificity for the Diagnosis of Mantle Cell Lymphoma Carol D. Jones, Katherine H. Darnell, Roger A.
High-Quality Genotyping Data from Formalin-Fixed, Paraffin-Embedded Tissue on the Drug Metabolizing Enzymes and Transporters Plus Array  Hanneke I. Vos,

High-Quality Genotyping Data from Formalin-Fixed, Paraffin-Embedded Tissue on the Drug Metabolizing Enzymes and Transporters Plus Array  Hanneke I. Vos,
Evaluation and Validation of Total RNA Extraction Methods for MicroRNA Expression Analyses in Formalin-Fixed, Paraffin-Embedded Tissues  Martina Doleshal,

Evaluation and Validation of Total RNA Extraction Methods for MicroRNA Expression Analyses in Formalin-Fixed, Paraffin-Embedded Tissues  Martina Doleshal,
Anna Sapino, Paul Roepman, Sabine C. Linn, Mireille H. J

Anna Sapino, Paul Roepman, Sabine C. Linn, Mireille H. J
Evaluation and Validation of Total RNA Extraction Methods for MicroRNA Expression Analyses in Formalin-Fixed, Paraffin-Embedded Tissues  Martina Doleshal,

Evaluation and Validation of Total RNA Extraction Methods for MicroRNA Expression Analyses in Formalin-Fixed, Paraffin-Embedded Tissues  Martina Doleshal,
Profiling and Discovery of Novel miRNAs from Formalin-Fixed, Paraffin-Embedded Melanoma and Nodal Specimens  Zhihai Ma, Weng-Onn Lui, Andrew Fire, Soheil.

Profiling and Discovery of Novel miRNAs from Formalin-Fixed, Paraffin-Embedded Melanoma and Nodal Specimens  Zhihai Ma, Weng-Onn Lui, Andrew Fire, Soheil.
Genotyping of DNA Samples Isolated from Formalin-Fixed Paraffin-Embedded Tissues Using Preamplification  Renee Baak-Pablo, Vincent Dezentje, Henk-Jan.

Genotyping of DNA Samples Isolated from Formalin-Fixed Paraffin-Embedded Tissues Using Preamplification  Renee Baak-Pablo, Vincent Dezentje, Henk-Jan.
Sensitive Detection of KRAS Mutations in Archived Formalin-Fixed Paraffin-Embedded Tissue Using Mutant-Enriched PCR and Reverse-Hybridization  Christoph.

Sensitive Detection of KRAS Mutations in Archived Formalin-Fixed Paraffin-Embedded Tissue Using Mutant-Enriched PCR and Reverse-Hybridization  Christoph.
RT-PCR Analysis of RNA Extracted from Bouin-Fixed and Paraffin-Embedded Lymphoid Tissues  Annunziata Gloghini, Barbara Canal, Ulf Klein, Luigino Dal Maso,

RT-PCR Analysis of RNA Extracted from Bouin-Fixed and Paraffin-Embedded Lymphoid Tissues  Annunziata Gloghini, Barbara Canal, Ulf Klein, Luigino Dal Maso,
Application of Selected Reaction Monitoring for Multiplex Quantification of Clinically Validated Biomarkers in Formalin-Fixed, Paraffin-Embedded Tumor.

Application of Selected Reaction Monitoring for Multiplex Quantification of Clinically Validated Biomarkers in Formalin-Fixed, Paraffin-Embedded Tumor.
The Frequency of Immunoglobulin Heavy Chain Gene and T-Cell Receptor γ-Chain Gene Rearrangements and Epstein-Barr Virus in ALK+ and ALK− Anaplastic Large.

The Frequency of Immunoglobulin Heavy Chain Gene and T-Cell Receptor γ-Chain Gene Rearrangements and Epstein-Barr Virus in ALK+ and ALK− Anaplastic Large.
Layered Peptide Array for Multiplex Immunohistochemistry

Layered Peptide Array for Multiplex Immunohistochemistry
Natalia Kholod, Jacques Boniver, Philippe Delvenne 

Natalia Kholod, Jacques Boniver, Philippe Delvenne 
Hybrid Model Integrating Immunohistochemistry and Expression Profiling for the Classification of Carcinomas of Unknown Primary Site  Barbara A. Centeno,

Hybrid Model Integrating Immunohistochemistry and Expression Profiling for the Classification of Carcinomas of Unknown Primary Site  Barbara A. Centeno,
A Quantitative Reverse Transcriptase-Polymerase Chain Reaction Assay to Identify Metastatic Carcinoma Tissue of Origin  Dimitri Talantov, Jonathan Baden,

A Quantitative Reverse Transcriptase-Polymerase Chain Reaction Assay to Identify Metastatic Carcinoma Tissue of Origin  Dimitri Talantov, Jonathan Baden,
Whole Genome Amplification for Array Comparative Genomic Hybridization Using DNA Extracted from Formalin-Fixed, Paraffin-Embedded Histological Sections 

Whole Genome Amplification for Array Comparative Genomic Hybridization Using DNA Extracted from Formalin-Fixed, Paraffin-Embedded Histological Sections 
Molecular Diagnosis of Necrotizing Fasciitis by 16S rRNA Gene Sequencing and Superantigen Gene Detection  Kenneth L. Muldrew, Jean F. Simpson, Charles.

Molecular Diagnosis of Necrotizing Fasciitis by 16S rRNA Gene Sequencing and Superantigen Gene Detection  Kenneth L. Muldrew, Jean F. Simpson, Charles.
Comparison of Snap Freezing versus Ethanol Fixation for Gene Expression Profiling of Tissue Specimens  Mark A. Perlmutter, Carolyn J.M. Best, John W.

Comparison of Snap Freezing versus Ethanol Fixation for Gene Expression Profiling of Tissue Specimens  Mark A. Perlmutter, Carolyn J.M. Best, John W.
Cold-Temperature Plastic Resin Embedding of Liver for DNA- and RNA-Based Genotyping  Sydney D. Finkelstein, Rajiv Dhir, Mordechai Rabinovitz, Michelle.

Cold-Temperature Plastic Resin Embedding of Liver for DNA- and RNA-Based Genotyping  Sydney D. Finkelstein, Rajiv Dhir, Mordechai Rabinovitz, Michelle.

Similar presentations

Ads by Google