1. Single-cell characterization of ovarian spheroids
David Morse, PhD student
Harvard University, Weitz Lab
University of Cambridge, Knowles Lab

2. Ovarian cancer spheroids

3. Single-cell sequencing
Bulk Sequencing
Microfluidic-enabled single-cell profiling
Single-cell
Sequencing

4. Bulk Sequencing
Single-cell
Sequencing

5. Radial-spatial resolution
Fluorescence
Cell count
Fluorescent distribution within a spheroid
200 µm

6. A pipeline for drug development
Enable the design of effective drug cocktails and combination therapies
Establish a method for examining context dependent single-cell gene expression
cultured spheres were resistant to many common chemotherapeutics, most notably
proteasome inhibitors, but were highly sensitive to the pro-oxidant elesclomol
broad downregulation of
genes involved in cell cycle signaling