1. Data quantification and normalization
M. truncatula
BSO treatment
Control
(h)GSH-depleted
Inoculation with S. meliloti
Time course
(0, 12, 24,…, 96h)
Time course
(0, 12, 24,…, 96h)
cDNA-AFLP analysis
6149 tags visualized
Data quantification and normalization
Group A:
299 nodulation regulated genes
CVnod
≥ 0.4
Control/(h)GSH-depleted
variance test p<0.05
Group B:
181 (h)GSH-regulated genes
Figure S1: Experimental design and data analysis scheme for isolation of (h)GSH-regulated genes during the nodulation process. Group A: the Coefficient of Variance (CVnod) ≥ 0.4, was retained to select genes differentially expressed during the nodulation process. Group B : analysis of variance (P≤0.05), was performed to select genes affected by the (h)GSH depletion