1. Pathology with Paper and Pencil
[Ref: P. Mandal, R. Dey, S. Chakraborty, Lab Chip, 2012, 12, ]
Normal filter paper
Filter paper soaked in photoresist; prebaked
Mask for UV exposure
Paper channel 1 1

2. Fabrication of Complex Structures on Paper
Zigzag design to investigate mixing
H-channel designed to investigate blood cell separation

3. Colorimetric Detection of Blood Glucose and Multiplexing
Calibration curve with Standard glucose
Calibration with standard glucose samples
Colorimetric Detection of Blood Glucose and Multiplexing
Blood sample to source pad
Multiplexing:
Simultaneous detection of multiple analytes like glucose and protein etc.
Blood group Identification
Protein assay
Anti A-B
Anti O
Glucose assay
Glucose detection process
Whatman grade 1
Length = 20 mm
W = 3 mm, Φ = 7 mm
Paper microchannel
Glucose Reagent
Antibody (ABO)
Reagents
Blood Sample
Plasma separated from RBC
Brown color
developed
Result Display
Separation
+ Reaction
Image Processing
Image Acquisition

4. Paper-based Kits for Antimicrobial Susceptibility Assays
Prototype:
Indian patent file no:
Working protocol:
Microfluidics and Microscale Transport Processes Laboratory

5. Towards a Commercializable Device
Functional block diagram of the med Colorimeter.
Paper Based Micro-fluidic Sensor with Blood Sample
Micro-controller/ Digital Signal Processor
LED based calibrated light source
Calibrated photo detector
Trans-impedance amplifier
+ filters
Analog to Digital Converter
Smart Phone
Bluetooth Low Energy Transceiver
A/Prof. Sudip Nag Prof. Suman Chakraborty

6. Prototype Design Paper Based Blood Sample Sensor Battery
Opaque Enclosure
Paper Based Blood Sample Sensor
Bluetooth Low Energy Transceiver
Calibrated LED with Driver and Optical Detector

7. Specification of the Prototype
S.N.
Parameters
Values 1
Principle of detection
Colorimetry 2
Wavelength/ illuminator
640 nm (red)/ LED 3
Intensity
13000 lux 4
Optical resolution of detector
0.01 lux 5
RF carrier/ protocol
2.4 GHz/ Bluetooth Low Energy 6
Digital resolution/ Speed of data transfer
23 bits/ 4 samples per second 7
App on smart phone
Custom developed 8
Supply voltage/ Power
3.0 V/ 50 mW typical

8. Acknowledgement Researchers and Students Faculty Member
Dr. Sudip Nag
Department of E&ECE
IIT Kharagpur, INDIA
Researchers and Students
Dr. Shantimoy Kar (former Ph.D student)
Sujay Biswas (current Ph.D student)

9. Acknowledgement Researchers and Students Dr. Shantimoy Kar
Sujay Biswas

10. Collaborative partners and funding Agencies