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Epithelial proteome profiling suggests the essential role of interferon-inducible proteins in patients with allergic rhinitis  Joseph Ndika, PhD, Liisa.

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Presentation on theme: "Epithelial proteome profiling suggests the essential role of interferon-inducible proteins in patients with allergic rhinitis  Joseph Ndika, PhD, Liisa."— Presentation transcript:

1 Epithelial proteome profiling suggests the essential role of interferon-inducible proteins in patients with allergic rhinitis  Joseph Ndika, PhD, Liisa Airaksinen, MD, PhD, Hille Suojalehto, MD, PhD, Piia Karisola, PhD, Nanna Fyhrquist, PhD, Anne Puustinen, PhD, Harri Alenius, PhD  Journal of Allergy and Clinical Immunology  Volume 140, Issue 5, Pages (November 2017) DOI: /j.jaci Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Journal of Allergy and Clinical Immunology 2017 140, 1288-1298DOI: (10
Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 1 Heat map of DA proteins across all groups. Hierarchical clustering of the 133 proteins found to be DA (FDR < 0.05) between the AS, AF, CS, and CF groups reveals the molecular profile of each group is unique, with the technical replicates being closest together. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig 2 Volcano plots (A) and overlapping DA proteins (B) in specified contrast sets are shown. The x-axis and y-axis of the volcano plot depict the log2 fold change and the −log of the t test P value, respectively. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig 3 Z score–normalized intensities of DA proteins common between the AS/AF and CS/CF contrast sets. IFIT1, IFIT3, and CST1 were found to be DA between seasons in patients with SAR and control subjects. However, although they are upregulated during the pollen season and downregulated outside the pollen season in patients with SAR, the reverse is true in control subjects. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

6 Fig 4 Genemania: analysis of DA proteins in all contrast sets. Red lines denote physical interactions between proteins, and blue lines indicate colocalization between proteins. Inner circle (stripes) shows pasted DA proteins, and outer circle shows associated proteins inferred from the literature. Colored circles refer to 3 most significant functions associated with the proteins. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

7 Fig 5 EnrichR clustergram of the most significant comparisons. Clusters of proteins to the 10 top enriched Gene Ontology biological pathway terms are shown. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

8 Fig 6 Western blot validation of MX1 and PRTN3. A, LC-MS/MS quantified Log2 intensities from in silico pools of 10 subjects across 3 replicates for both MX1 and PRTN3. B, Technical validation of MS quantitation by means of Western blotting. Data points are normalized Western blot intensities (means ± SEMs) individually measured from the same 10 subjects per group used for MS analysis. C, Functional validation of MX1 and PRTN3 abundance levels by means of Western blot analysis of the 20 MS-quantified samples plus an additional 40 samples, making up a total of 60 subjects. Error bars are means ± SEMs of 29 patients with SAR or 31 control subjects. In both Fig 6, B and C, Western blot intensities are normalized to total protein content in each sample and band intensity of a pooled sample loaded in each gel. *P < .05, **P < .01, and ***P < .001. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

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