1. Diversity of Life – Activities 4 & 6 Atmosphere and Ocean Circulation – Activities 9 & 10
Rain Alternative
Week of April 16th 2018
Version 1.1.
4/19/2018 8:27:08 AM

3. Activity 4 – Leaf Litter Organisms – Set Up
Diversity of Life –
Activity 4 – Leaf Litter Organisms – Set Up

4. Assemble Berlese funnel apparatus
Goose-neck lamp
Place tape on collecting container portion. Use permanent marker to label tape w/ lab section number and group number.
Use graduated cylinder to add isopropyl alcohol to the bottom of the collecting container until it reaches a depth of approximately 1 cm.
Place funnel on top of collecting container (Fig 4.1.)
Make sure that the narrow end of the funnel is covered with screen.
Empty contents of the plastic collecting bag into funnel. Funnel should be as full as possible.
Leaf litter
Funnel
Funnel support/collection container
Screen cover
~1 cm isopropyl alcohol
Figure 4.1. Berlese funnel apparatus. Large funnel w/ leaf litter supported in collection container w/ ~1 cm isopropyl alcohol.

5. Activity 6 – Microscopic Fungi – Set Up
Diversity of Life -
Activity 6 – Microscopic Fungi – Set Up

6. Microscopic fungi Important decomposers in most ecosystems.
Reproduce via spores too small to see with unaided eye, yet present on almost every surface.
Inoculating petri dishes with fungal spores will enable you to see the fungi after they grow on the petri dish.

7. Get two Petri dishes with starch agar.
Handle Petri dishes carefully to minimize contamination.

8. Use marker to label bottom Petri dishes (keep lids on).
control
loc 1
loc 2
control
loc 3
loc 4
Figures 6.1 and 6.2. Markings and treatment labels for bottom of starch agar Petri dishes #1 and #2.
Use marker to label bottom Petri dishes (keep lids on).
Include Lab section #, Group # and Petri dish # (1 or 2).
Draw lines that divide Petri dish into 3 pie-shaped sections and label sections.

9. Sampling microscopic fungi
Select 4 different surfaces from 4 locations in your quadrat.
Use separate sterile cotton swab for each surface.
Only remove one swab at a time to maintain sterility.
After swabbing surface, touch cotton tip to agar in appropriate area of petri dish.
DO NOT seal Petri Dishes with parafilm.

10. Table 6.1. Descriptions of four microscopic fungi surfaces
Description of
Location #
Location
Surface 1 X 2 3 4
Record surface type for each of your four samples in Table 6.1.

11. Atmosphere and Ocean Circulation - Activity 9 – Ocean Surface Currents

12. Ocean Surface Circulation Currents
Lateral movement of water at ocean surface.
Determined by combined influence of atmospheric circulation patterns, water density differences, continents and the rotation of the Earth.
Important due to role in influencing climate, global biogeochemical cycles and transporting organisms, chemicals, nutrients and pollutants.
Image © Rick Lumpkin NOAA/OAML.

13. Recreate map and plot data for eight buoys.
Table 9.1. Ocean drifter buoy position coordinates (latitude, longitude) by date and buoy number for eight buoys
67906
71369
67903
Date
Lat
Lon
11/10/07 39
148
11/05/07 21
-130
10/22/07 46
170
12/10/07 40
150
12/05/07 23
-138
11/22/07
174
01/10/08 38
157
01/05/08 22
-141
12/22/07
178
02/10/08 37
163
02/05/08
-146
01/22/08 45
-177
03/10/08 35
169
03/05/08
-150
02/22/08
-170
04/10/08
172
04/05/08 24
-156
03/22/08
-166
05/10/08 36
173
05/05/08
-160
04/22/08
-162
Figure 9.2. Map of ocean drifter buoy tracks for eight buoys in the Pacific Ocean for periods of 6 to 20 months for various years. (Source: NOAA).
Recreate map and plot data for eight buoys.
Connect points, add arrow to show direction, label buoy lines.

14. Atmosphere and Ocean Circulation –
Activity 10 – Vertical Structure of the Ocean

15. Mixed zone Thermocline Deep zone
Uniformly mixed water, i.e. little to no change in temperature with increased depth.
Thermocline
Temperature decreases rapidly with increased depth.
Deep zone
Water is uniformly cold, i.e. little to no change in temperature with increased depth.

16. Typical Temperature Profiles

17. Ocean temperature (°C)
Table 10.1 Ocean temperature (°C) data from surface to depth of 600 m at 5° N 110° W
Table 10.2 Ocean temperature (°C) data from surface to depth of 600 m at 5° N 140° W
Table 10.3 Ocean temperature (°C) data from surface to depth of 600 m at 5° N 180° W
Ocean temperature (°C)
Temp(°C)
Depth (m)
26.0
25.5 25
22.5 50
18.0 75
13.5
100
13.0
125
12.0
150
11.0
200
10.5
250
10.0
300
9.5
350
9.0
400
8.5
450 8
500
Temp(°C)
Depth (m)
26.0 25 50 75
25.5
100
23.0
125
18.0
150
13.5
175
13.0
200
11.0
250
10.0
300
9.0
350
8.5
400
8.0
450
7.5
500
Temp(°C)
Depth (m)
28.5 25 50
28.0 75
27.5
100
21.5
150
13.5
200
10.5
250
9.5
300
9.0
350
8.5
400
8.0
450
7.5
500
Ocean depth (m)
Figure Ocean temperature (°C) from surface to a depth of 500 m for three longitudes (110° W, 140° W, 180° W) at 5°N latitude.
Plot temperature vs. depth data for locations from three locations.

18. Table Starting and ending depths (m) for mixed, thermocline and deep zone at three sampling locations
Sampling Location Longitude at 5° N Latitude
110° W
140° W
180°9 W
Zone
Start/End
Depth
Mixed zone
Start
End
Thermocline
Deep zone
500
Determine beginning and ending depths for each zone at all three locations, based on your graph.

19. What’s Due Weekly Data Sheets: Weekly Write-Ups:
P. 161, 355, 363 and 367.
P. 147, 165, and 371.
PowerPoint available at