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1. Pure Protein (0.3 mL, mM; ~ 10 mg)

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Presentation on theme: "1. Pure Protein (0.3 mL, mM; ~ 10 mg)"— Presentation transcript:

1 Solution Structure Determination by NMR and Distance Geometry Calculations
1. Pure Protein (0.3 mL, mM; ~ 10 mg) 2. Sequence-specific resonance assignments 3. Sequence-specifice distance constraints disulfide bonds spin-spin coupling constants internuclear distance constraints from NOEs residual dipolar couplings hydrogen bonds standard bond lengths; bond angles 4. Distance Geometry Calculations 5. Constrainted Energy Minimization; Refinement

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22 Solution Structure Determination by NMR and Distance Geometry Calculations
1. Pure Protein (0.3 mL, mM; ~ 10 mg) 2. Sequence-specific resonance assignments 3. Sequence-specifice distance constraints disulfide bonds spin-spin coupling constants internuclear distance constraints from NOEs residual dipolar couplings hydrogen bonds standard bond lengths; bond angles 4. Distance Geometry Calculations 5. Constrainted Energy Minimization; Refinement

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