1. Volume 98, Issue 10, Pages 2063-2071 (May 2010)
Altered Spatiotemporal Dynamics of the Mitochondrial Membrane Potential in the Hypertrophied Heart 
Hongwei Jin, Robert D. Nass, Paul J. Joudrey, Alexander R. Lyon, Elie R. Chemaly, Kleopatra Rapti, Fadi G. Akar 
Biophysical Journal 
Volume 98, Issue 10, Pages (May 2010)
DOI: /j.bpj
Copyright © 2010 Biophysical Society Terms and Conditions

2. Figure 1
(A) Three representative traces (A–C) from a total of 6400 pixels are shown indicating the measurement of background fluorescence used to monitor relative changes in ΔΨm. Also shown is the background fluorescence during and after (for up to 300 min) the dye-loading and washout procedures. These data demonstrate the stability of TMRM fluorescence during steady-state perfusion. The steady-state level of TMRM fluorescence for each pixel is used for normalization purposes. (B) Representative ECG images of a sham-operated and an aortic-banded LVH heart. H&E-stained histological sections from sham-operated and LVH hearts indicate marked concentric hypertrophy in aortic banded animals. Also shown are representative optical AP traces measured in representative di-4-ANEPPS-stained normal and LVH hearts during steady-state pacing.
Biophysical Journal  , DOI: ( /j.bpj )
Copyright © 2010 Biophysical Society Terms and Conditions

3. Figure 2
(A) Average and SD of ΔΨm response to IR injury in normal and LVH hearts. Clearly, LVH prevents ischemia-induced depolarization of ΔΨm. (B) Average ΔΨm response during the entire episode of ischemia (over 7 min) and reperfusion in normal and LVH hearts. LVH is associated with a significantly more polarized ΔΨm during both ischemia and reperfusion.
Biophysical Journal  , DOI: ( /j.bpj )
Copyright © 2010 Biophysical Society Terms and Conditions

4. Figure 3
Contour maps showing the spatiotemporal distribution of ΔΨm during the course of ischemia (top row) and reperfusion (bottom row) in representative normal (A) and LVH (B) hearts.
Biophysical Journal  , DOI: ( /j.bpj )
Copyright © 2010 Biophysical Society Terms and Conditions

5. Figure 4
ΔΨm contour maps recorded after 7 min of ischemia in representative normal (A) and LVH (B) hearts. (Right) Multiple traces recorded from the same normal and LVH hearts during the course of IR injury.
Biophysical Journal  , DOI: ( /j.bpj )
Copyright © 2010 Biophysical Society Terms and Conditions

6. Figure 5
(A) Spatial heterogeneity indexed by the SD of ΔΨm in normal and LVH hearts. (B) Average and SD of ΔΨm heterogeneity measured during ischemia and reperfusion in normal and LVH hearts.
Biophysical Journal  , DOI: ( /j.bpj )
Copyright © 2010 Biophysical Society Terms and Conditions

7. Figure 6
AS as an index of spontaneous arrhythmias measured during ischemia and reperfusion from all unpaced normal and LVH hearts. Representative ECG traces indicate progressively more complex arrhythmias, ranging from AS 0 to 5. AS: arrhythmia score.
Biophysical Journal  , DOI: ( /j.bpj )
Copyright © 2010 Biophysical Society Terms and Conditions

8. Figure 7
Incidence of VT/VF in paced hearts during IR injury. Representative volume-conducted ECG traces measured at baseline, during early and late ischemia, and after reperfusion in control (left) and LVH (right) hearts. VT/VF was observed exclusively in paced LVH hearts. Representative AP traces from control and LVH hearts demonstrate rapid shortening of APD in LVH but not control hearts in response to 7 min of ischemia (bottom).
Biophysical Journal  , DOI: ( /j.bpj )
Copyright © 2010 Biophysical Society Terms and Conditions