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Both patient 1 and her family members showed a normal physiological AQP2 response to DDAVP. Immunoblotting for AQP2 was performed on urinary exosomes derived from urine collected before (−) and after (+) administration of DDAVP. Murine immortalized cortical... Both patient 1 and her family members showed a normal physiological AQP2 response to DDAVP. Immunoblotting for AQP2 was performed on urinary exosomes derived from urine collected before (−) and after (+) administration of DDAVP. Murine immortalized cortical collecting duct cells cultured in the presence or absence of DDAVP served as controls. Representative blots showing AQP2 response to DDAVP administration in a heterozygous family member as well as patient 1 are shown. WT, wild type. Ernie M.H.F. Bongers et al. JASN 2017;28: ©2017 by American Society of Nephrology
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