1. Differential regulation of SRF and NFAT by Ca2+.
Differential regulation of SRF and NFAT by Ca2+. (A) SRF (solid diamonds) or NFAT (open circles) transcriptional activity was determined in WT DT40 cells stimulated with the indicated concentrations of ionomycin along with 5 ng/ml PMA. (B) CN is a downstream effector of Ca2+ in the regulation of SRF by BCR signaling. SRF or NFAT transcriptional activity was determined in WT DT40 cells pre‐treated with the indicated concentrations of the CN inhibitor CsA prior to stimulation with anti‐IgM. The activity of the non‐stimulated and anti‐IgM stimulated cells were set as 0 and 100% activation respectively. (C) SRF transcriptional activity was determined in IP3R−/− DT40 cells pre‐treated with 400 nM CsA prior to stimulation with anti‐IgM. (D) SRF transcriptional activity was determined in IP3R−/− DT40 cells transfected with 15 μg empty vector or 10 or 15 μg CN‐A and stimulated with 5 ng/ml PMA.
Shengli Hao et al. EMBO J. 2003;22:
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