Presentation is loading. Please wait.

Presentation is loading. Please wait.

D-tryptophan from probiotic bacteria influences the gut microbiome and allergic airway disease  Inge Kepert, PhD, Juliano Fonseca, PhD, Constanze Müller,

Published byJakob Fiedler Modified over 6 years ago

Similar presentations

Presentation on theme: "D-tryptophan from probiotic bacteria influences the gut microbiome and allergic airway disease  Inge Kepert, PhD, Juliano Fonseca, PhD, Constanze Müller,"— Presentation transcript:

1 D-tryptophan from probiotic bacteria influences the gut microbiome and allergic airway disease 
Inge Kepert, PhD, Juliano Fonseca, PhD, Constanze Müller, PhD, Katrin Milger, MD, Kerstin Hochwind, PhD, Matea Kostric, MSc, Maria Fedoseeva, MSc, Caspar Ohnmacht, PhD, Stefan Dehmel, PhD, Petra Nathan, PhD, Sabine Bartel, PhD, Oliver Eickelberg, MD, Michael Schloter, PhD, Anton Hartmann, PhD, Philippe Schmitt-Kopplin, PhD, Susanne Krauss-Etschmann, MD  Journal of Allergy and Clinical Immunology  Volume 139, Issue 5, Pages (May 2017) DOI: /j.jaci Copyright © Terms and Conditions

2 Fig 1 Screening of supernatants from different probiotic strains for immune activity on human cells. A, Dose-dependent capacity of bacterial supernatants from LGG (fx1), Bifidobacterium BB-420 (fx2), and Lactobacillus casei W56 (fx3) to lower CCL17 secretion of human Hodgkin lymphoma KM-H2 cells. The negative control was nonprobiotic Lactobacillus rhamnosus DSM (fx4). Three independent experiments in duplicates are shown (mean ± SD percentages relative to CCL17 secretion of untreated KM-H2 cells). LGG: **P < .005 and ***P < .0005, L casei W56: ##P < .005, ###P < .0005, BB-420: §§P < .005, §§§P < .0005, Student t test. B, Capacity of supernatants from LGG, Bifidobacterium BB-420, Lactobacillus casei W56, or nonprobiotic Lactobacillus rhamnosus DSM to prevent full upregulation of costimulatory molecules and HLA-DR on LPS-stimulated human monocyte-derived DCs. +/-, With/without bacterial supernatant. Five independent experiments are shown (mean ± SD percentages relative to LPS alone). **P < .01 and ***P < .001, Dunn multiple comparison test. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © Terms and Conditions

3 Fig 2 Overview on the ability of bacterial supernatants from all 37 strains to decrease CCL17 secretion of KM-H2 cells. Shaded bars, Nonprobiotic Lactobacillus rhamnosus DSM (negative control); LGG was included as a positive control in all experiments with strains other than lactobacilli. Open bars, Untreated KM-H2 cells and medium control cells. Three independent experiments in duplicates are shown (mean + SD percentages relative to CCL17 secretion of untreated KM-H2 cells). **P < .005 and ***P < .0005, Student t test. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © Terms and Conditions

4 Fig 3 Capacity of subfractions of probiotic supernatants to decrease CCL17 secretion in KM-H2 cells. Subfractions with different polarity (MeOH/H2O gradient chromatography) from supernatants of LGG (top) or Lactobacillus casei W56 (middle). Negative controls were nonprobiotic DSM and blank CDM1 medium (bottom). Three independent experiments in duplicates are shown (mean ± SD percentages relative to constitutive CCL17 secretion of untreated KM-H2 cells). **P < .005 and ***P < .0005, Student t test. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © Terms and Conditions

5 Fig 4 Effect of tryptophan L- and D-isomers on CCL17 secretion by KM-H2 cells. KM-H2 cells were stimulated with different concentrations of synthetic L- and D-isomers of tryptophan followed by CCL17 quantification in KM-H2 culture medium after 24 hours. Circles, D-tryptophan; diamonds, L-tryptophan. Three independent experiments in duplicates are shown (mean ± SD percentages relative to constitutive CCL17 secretion of untreated KM-H2 cells). *P < .05, **P < .005, and ***P < .0005, Student t test. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © Terms and Conditions

6 Fig 5 Oral D-tryptophan reduces allergic airway inflammation. A, Serum D-tryptophan in mice receiving D-tryptophan (50 mmol/L) in drinking water or water only (ultraperformance liquid chromatography mass spectrometry peak areas). Note the different scales for D-tryptophan (solid bars) and L-tryptophan (shaded bars). **P = .006 and ***P = .004, Welch Test, mean ± SD. B, Total number of cells in bronchoalveolar lavage fluid (BAL). C, Differential cell count. D, Measurement of airway resistance to increasing doses of methacholine (2-way ANOVA with the Bonferroni posttest). E, Geometric mean (fold change) of Ifn-g and Il-4 in lung-derived CD3+CD4+ lymphocytes. F, Il-4 levels in bronchoalveolar lavage of mice, as assessed by using a Cytometric Bead Array. G, Helios-positive Treg cells of lung-derived CD3+CD4+Foxp3+ lymphocytes. Student t test: *P < .05, **P < .01, and ***P < Fig 5, B, C, E and F, n = 8 mice per group, Mann-Whitney U test, median ± SD, *P < .05, ***P < .001, and Fig 5, D and G, n = 6 to 12 mice per group. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © Terms and Conditions

7 Fig 6 D-tryptophan (DTrp) influences in vitro primary T-cell differentiation. Primary murine splenocytes were differentiated toward TH1 (A), TH2 (B), and Treg (C) cells with respective cytokine mixes in the presence of 0, 10, or 50 μmol/L D-tryptophan (dissolved in water). Differentiation was assessed by means of flow cytometry, quantitative RT-PCR, and the Cytometric Bead Array for Il-13 and Il-5 protein levels from culture supernatants. Graphs depict fold changes to differentiated cells not treated with D-tryptophan. *P < .05, n = 3 to 4 independent experiments, Mann-Whitney U test. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © Terms and Conditions

8 Fig 7 Oral D-tryptophan (DTrp) supplementation increased gut Treg cell numbers and the intestinal bacterial community in mice with AAI. A, Percentage of Foxp3+ cells within CD3+CD4+ T cells in the lamina propria of the colon. ****P < .0001, n = 6 to 12 mice per group, Student t test. B, α-Diversity of bacterial communities. Shannon diversity index was used to estimate bacterial diversity for each treatment (Wilcoxon rank sum test). Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © Terms and Conditions

9 Fig E1 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © Terms and Conditions

10 Fig E2 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © Terms and Conditions

11 Fig E3 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © Terms and Conditions

12 Fig E4 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © Terms and Conditions

13 Fig E5 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © Terms and Conditions

14 Fig E6 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © Terms and Conditions

15 Fig E7 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © Terms and Conditions

16 Fig E8 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © Terms and Conditions

17 Fig E9 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © Terms and Conditions

Download ppt "D-tryptophan from probiotic bacteria influences the gut microbiome and allergic airway disease  Inge Kepert, PhD, Juliano Fonseca, PhD, Constanze Müller,"

Similar presentations

Multiple-checkpoint inhibition of thymic stromal lymphopoietin–induced TH2 response by TH17-related cytokines  Sofia I. Bogiatzi, BSc, Maude Guillot-Delost,

Multiple-checkpoint inhibition of thymic stromal lymphopoietin–induced TH2 response by TH17-related cytokines  Sofia I. Bogiatzi, BSc, Maude Guillot-Delost,
Vitamin D3 treatment of vitamin D–insufficient asthmatic patients does not alter immune cell function  Brandy Reid, MS, Pierre-Olivier Girodet, MD, PhD,

Vitamin D3 treatment of vitamin D–insufficient asthmatic patients does not alter immune cell function  Brandy Reid, MS, Pierre-Olivier Girodet, MD, PhD,
Immunomodulatory effects of aqueous birch pollen extracts and phytoprostanes on primary immune responses in vivo  Jan Gutermuth, MD, Mayte Bewersdorff,

Immunomodulatory effects of aqueous birch pollen extracts and phytoprostanes on primary immune responses in vivo  Jan Gutermuth, MD, Mayte Bewersdorff,
Barbara Stanic, PhD, Willem van de Veen, PhD, Oliver F

Barbara Stanic, PhD, Willem van de Veen, PhD, Oliver F
Topical application of a vitamin D3 analogue and corticosteroid to psoriasis plaques decreases skin infiltration of TH17 cells and their ex vivo expansion 

Topical application of a vitamin D3 analogue and corticosteroid to psoriasis plaques decreases skin infiltration of TH17 cells and their ex vivo expansion 
Regulation of TH2 responses by the pulmonary Clara cell secretory 10-kd protein  Chih-Hsing Hung, MD, Li-Chen Chen, MD, Zhongjian Zhang, PhD, Bhabadeb.

Regulation of TH2 responses by the pulmonary Clara cell secretory 10-kd protein  Chih-Hsing Hung, MD, Li-Chen Chen, MD, Zhongjian Zhang, PhD, Bhabadeb.
CD4+CD25+ regulatory T cells suppress contact hypersensitivity reactions through a CD39, adenosine-dependent mechanism  Sabine Ring, PhD, Stephen J. Oliver,

CD4+CD25+ regulatory T cells suppress contact hypersensitivity reactions through a CD39, adenosine-dependent mechanism  Sabine Ring, PhD, Stephen J. Oliver,
Induction of anergic allergen-specific suppressor T cells using tolerogenic dendritic cells derived from children with allergies to house dust mites 

Induction of anergic allergen-specific suppressor T cells using tolerogenic dendritic cells derived from children with allergies to house dust mites 
Maternal immune response to helminth infection during pregnancy determines offspring susceptibility to allergic airway inflammation  Kathrin Straubinger,

Maternal immune response to helminth infection during pregnancy determines offspring susceptibility to allergic airway inflammation  Kathrin Straubinger,
Ambient particulate matter directs nonclassic dendritic cell activation and a mixed TH1/TH2-like cytokine response by naive CD4+ T cells  Marc A. Williams,

Ambient particulate matter directs nonclassic dendritic cell activation and a mixed TH1/TH2-like cytokine response by naive CD4+ T cells  Marc A. Williams,
The activating protein 1 transcription factor basic leucine zipper transcription factor, ATF- like (BATF), regulates lymphocyte- and mast cell–driven immune.

The activating protein 1 transcription factor basic leucine zipper transcription factor, ATF- like (BATF), regulates lymphocyte- and mast cell–driven immune.
Let-7 microRNA–mediated regulation of IL-13 and allergic airway inflammation  Manish Kumar, MSc, Tanveer Ahmad, MSc, Amit Sharma, PhD, Ulaganathan Mabalirajan,

Let-7 microRNA–mediated regulation of IL-13 and allergic airway inflammation  Manish Kumar, MSc, Tanveer Ahmad, MSc, Amit Sharma, PhD, Ulaganathan Mabalirajan,
The activating protein 1 transcription factor basic leucine zipper transcription factor, ATF- like (BATF), regulates lymphocyte- and mast cell–driven immune.

The activating protein 1 transcription factor basic leucine zipper transcription factor, ATF- like (BATF), regulates lymphocyte- and mast cell–driven immune.
IgE cross-linking impairs monocyte antiviral responses and inhibits influenza-driven TH1 differentiation  Regina K. Rowe, MD, PhD, David M. Pyle, MD,

IgE cross-linking impairs monocyte antiviral responses and inhibits influenza-driven TH1 differentiation  Regina K. Rowe, MD, PhD, David M. Pyle, MD,
Sytze de Roock, PhD, Arie J

Sytze de Roock, PhD, Arie J
Reduced IFN-γ receptor expression and attenuated IFN-γ response by dendritic cells in patients with atopic dermatitis  Eva Gros, MSc, Susanne Petzold,

Reduced IFN-γ receptor expression and attenuated IFN-γ response by dendritic cells in patients with atopic dermatitis  Eva Gros, MSc, Susanne Petzold,
Impaired barrier function in patients with house dust mite–induced allergic rhinitis is accompanied by decreased occludin and zonula occludens-1 expression 

Impaired barrier function in patients with house dust mite–induced allergic rhinitis is accompanied by decreased occludin and zonula occludens-1 expression 
Interferon response factor 3 is essential for house dust mite–induced airway allergy  Thomas Marichal, DVM, Denis Bedoret, DVM, PhD, Claire Mesnil, DVM,

Interferon response factor 3 is essential for house dust mite–induced airway allergy  Thomas Marichal, DVM, Denis Bedoret, DVM, PhD, Claire Mesnil, DVM,
Frank Kirstein, PhD, Natalie E

Frank Kirstein, PhD, Natalie E
Nazanin Farhadi, MSc, Laura Lambert, BA, Chiara Triulzi, PhD, Peter J

Nazanin Farhadi, MSc, Laura Lambert, BA, Chiara Triulzi, PhD, Peter J

Similar presentations

Ads by Google